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| | <StructureSection load='5ebd' size='340' side='right'caption='[[5ebd]], [[Resolution|resolution]] 2.60Å' scene=''> | | <StructureSection load='5ebd' size='340' side='right'caption='[[5ebd]], [[Resolution|resolution]] 2.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5ebd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EBD OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5EBD FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5ebd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EBD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EBD FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3x3m|3x3m]], [[3x3n|3x3n]], [[5ebc|5ebc]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">eccB1, Rv3869 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ebd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ebd OCA], [https://pdbe.org/5ebd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ebd RCSB], [https://www.ebi.ac.uk/pdbsum/5ebd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ebd ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5ebd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ebd OCA], [http://pdbe.org/5ebd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ebd RCSB], [http://www.ebi.ac.uk/pdbsum/5ebd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ebd ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ECCB1_MYCTU ECCB1_MYCTU]] Part of the ESX-1 specialized secretion system, which delivers several virulence factors to host cells during infection, including the key virulence factors EsxA (ESAT-6) and EsxB (CFP-10).<ref>PMID:16368961</ref> | + | [https://www.uniprot.org/uniprot/ECCB1_MYCTU ECCB1_MYCTU] Part of the ESX-1 specialized secretion system, which delivers several virulence factors to host cells during infection, including the key virulence factors EsxA (ESAT-6) and EsxB (CFP-10).<ref>PMID:16368961</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Myctu]] | + | [[Category: Mycobacterium tuberculosis H37Rv]] |
| - | [[Category: Bi, L J]] | + | [[Category: Bi LJ]] |
| - | [[Category: Li, D F]] | + | [[Category: Li DF]] |
| - | [[Category: Qi, C]] | + | [[Category: Qi C]] |
| - | [[Category: Xie, X Q]] | + | [[Category: Xie XQ]] |
| - | [[Category: Zhang, X L]] | + | [[Category: Zhang XL]] |
| - | [[Category: Alpha-beta-alpha sandwich]]
| + | |
| - | [[Category: Atpase]]
| + | |
| - | [[Category: Beta-sheet]]
| + | |
| - | [[Category: Esx-1 secretion system]]
| + | |
| - | [[Category: Protein transport]]
| + | |
| - | [[Category: T7ss]]
| + | |
| Structural highlights
Function
ECCB1_MYCTU Part of the ESX-1 specialized secretion system, which delivers several virulence factors to host cells during infection, including the key virulence factors EsxA (ESAT-6) and EsxB (CFP-10).[1]
Publication Abstract from PubMed
The protein EccB1, a core component of the type VII secretion system (T7SS) of Mycobacterium tuberculosis, has been identified as an ATPase and is essential for the secretion of virulence factors by the ESX-1 system. In a previous study, EccB1 structures were determined in two different conformations. Here, two new conformations are identified and described. These four conformations present snapshots of the swinging movement of the membrane-distal domain A2. The movement of this domain involves conformational changes in two flexible loops (loop A, residues 243-264, and loop B, residues 324-341) which are rich in proline and glycine residues and connect domain A2 to domains C1 and B2. It is proposed that the movement of this domain is related to the ATPase activity of EccB1 and its homologues, as well as to the substrate transport of ESX secretion systems.
Crystallographic observation of the movement of the membrane-distal domain of the T7SS core component EccB1 from Mycobacterium tuberculosis.,Xie XQ, Zhang XL, Qi C, Li de F, Fleming J, Wang da C, Bi LJ Acta Crystallogr F Struct Biol Commun. 2016 Feb 1;72(Pt 2):139-44. doi:, 10.1107/S2053230X16000212. Epub 2016 Jan 22. PMID:26841765[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Brodin P, Majlessi L, Marsollier L, de Jonge MI, Bottai D, Demangel C, Hinds J, Neyrolles O, Butcher PD, Leclerc C, Cole ST, Brosch R. Dissection of ESAT-6 system 1 of Mycobacterium tuberculosis and impact on immunogenicity and virulence. Infect Immun. 2006 Jan;74(1):88-98. PMID:16368961 doi:10.1128/IAI.74.1.88-98.2006
- ↑ Xie XQ, Zhang XL, Qi C, Li de F, Fleming J, Wang da C, Bi LJ. Crystallographic observation of the movement of the membrane-distal domain of the T7SS core component EccB1 from Mycobacterium tuberculosis. Acta Crystallogr F Struct Biol Commun. 2016 Feb 1;72(Pt 2):139-44. doi:, 10.1107/S2053230X16000212. Epub 2016 Jan 22. PMID:26841765 doi:http://dx.doi.org/10.1107/S2053230X16000212
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