1lgt

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[[Image:1lgt.jpg|left|200px]]
[[Image:1lgt.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1lgt |SIZE=350|CAPTION= <scene name='initialview01'>1lgt</scene>, resolution 1.70&Aring;
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The line below this paragraph, containing "STRUCTURE_1lgt", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=BP3:2&#39;-CHLORO-BIPHENYL-2,3-DIOL'>BP3</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Biphenyl-2,3-diol_1,2-dioxygenase Biphenyl-2,3-diol 1,2-dioxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.11.39 1.13.11.39] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1lgt| PDB=1lgt | SCENE= }}
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|RELATEDENTRY=[[1han|1HAN]], [[1lkd|1LKD]], [[1kmy|1KMY]], [[1knd|1KND]], [[1knf|1KNF]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lgt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lgt OCA], [http://www.ebi.ac.uk/pdbsum/1lgt PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1lgt RCSB]</span>
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}}
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'''CRYSTAL STRUCTURE OF 2,3-DIHYDROXYBIPHENYL 1,2-DIOXYGENASE (DHBD) COMPLEXED WITH 2'-Cl DIHYDROXYBIPHENYL (DHB)'''
'''CRYSTAL STRUCTURE OF 2,3-DIHYDROXYBIPHENYL 1,2-DIOXYGENASE (DHBD) COMPLEXED WITH 2'-Cl DIHYDROXYBIPHENYL (DHB)'''
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[[Category: Dai, S.]]
[[Category: Dai, S.]]
[[Category: 2,3-dihydroxybiphenyl]]
[[Category: 2,3-dihydroxybiphenyl]]
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[[Category: anaerobic]]
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[[Category: Anaerobic]]
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[[Category: extradiol dioxygenase]]
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[[Category: Extradiol dioxygenase]]
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[[Category: non-heme iron]]
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[[Category: Non-heme iron]]
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[[Category: pcb biodegradation]]
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[[Category: Pcb biodegradation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:54:13 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:02:41 2008''
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Revision as of 20:54, 2 May 2008

Image:1lgt.jpg

Template:STRUCTURE 1lgt

CRYSTAL STRUCTURE OF 2,3-DIHYDROXYBIPHENYL 1,2-DIOXYGENASE (DHBD) COMPLEXED WITH 2'-Cl DIHYDROXYBIPHENYL (DHB)


Overview

The microbial degradation of polychlorinated biphenyls (PCBs) provides the potential to destroy these widespread, toxic and persistent environmental pollutants. For example, the four-step upper bph pathway transforms some of the more than 100 different PCBs found in commercial mixtures and is being engineered for more effective PCB degradation. In the critical third step of this pathway, 2,3-dihydroxybiphenyl (DHB) 1,2-dioxygenase (DHBD; EC 1.13.11.39) catalyzes aromatic ring cleavage. Here we demonstrate that ortho-chlorinated PCB metabolites strongly inhibit DHBD, promote its suicide inactivation and interfere with the degradation of other compounds. For example, k(cat)(app) for 2',6'-diCl DHB was reduced by a factor of approximately 7,000 relative to DHB, and it bound with sufficient affinity to competitively inhibit DHB cleavage at nanomolar concentrations. Crystal structures of two complexes of DHBD with ortho-chlorinated metabolites at 1.7 A resolution reveal an explanation for these phenomena, which have important implications for bioremediation strategies.

About this Structure

1LGT is a Single protein structure of sequence from Burkholderia sp.. Full crystallographic information is available from OCA.

Reference

Identification and analysis of a bottleneck in PCB biodegradation., Dai S, Vaillancourt FH, Maaroufi H, Drouin NM, Neau DB, Snieckus V, Bolin JT, Eltis LD, Nat Struct Biol. 2002 Dec;9(12):934-9. PMID:12415290 Page seeded by OCA on Fri May 2 23:54:13 2008

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OCA

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