8c9y
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | The | + | ==The MK-RSL - sulfonato-calix[8]arene complex, H32 form== |
| + | <StructureSection load='8c9y' size='340' side='right'caption='[[8c9y]], [[Resolution|resolution]] 1.18Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8c9y]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ralstonia_solanacearum Ralstonia solanacearum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8C9Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8C9Y FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.18Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BDF:BETA-D-FRUCTOPYRANOSE'>BDF</scene>, <scene name='pdbligand=EVB:sulfonato-calix[8]arene'>EVB</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8c9y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8c9y OCA], [https://pdbe.org/8c9y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8c9y RCSB], [https://www.ebi.ac.uk/pdbsum/8c9y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8c9y ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/D8NA05_RALSL D8NA05_RALSL] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Controlled protein assembly and crystallization is necessary as a means of generating diffraction-quality crystals as well as providing a basis for new types of biomaterials. Water-soluble calixarenes are useful mediators of protein crystallization. Recently, it was demonstrated that Ralstonia solanacearum lectin (RSL) co-crystallizes with anionic sulfonato-calix[8]arene (sclx(8)) in three space groups. Two of these co-crystals only grow at pH </= 4 where the protein is cationic, and the crystal packing is dominated by the calixarene. This paper describes a fourth RSL-sclx(8) co-crystal, which was discovered while working with a cation-enriched mutant. Crystal form IV grows at high ionic strength in the pH range 5-6. While possessing some features in common with the previous forms, the new structure reveals alternative calixarene binding modes. The occurrence of C(2)-symmetric assemblies, with the calixarene at special positions, appears to be an important result for framework fabrication. Questions arise regarding crystal screening and exhaustive searching for polymorphs. | ||
| - | + | Protein-macrocycle polymorphism: crystal form IV of the Ralstonia solanacearum lectin-sulfonato-calix[8]arene complex.,Mockler NM, Ramberg KO, Crowley PB Acta Crystallogr D Struct Biol. 2023 Jul 1;79(Pt 7):624-631. doi: , 10.1107/S2059798323003832. Epub 2023 Jun 14. PMID:37314405<ref>PMID:37314405</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 8c9y" style="background-color:#fffaf0;"></div> |
| - | [[Category: Crowley | + | == References == |
| - | [[Category: Mockler | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Ralstonia solanacearum]] | ||
| + | [[Category: Crowley PB]] | ||
| + | [[Category: Mockler NM]] | ||
| + | [[Category: Ramberg K]] | ||
Revision as of 07:17, 12 July 2023
The MK-RSL - sulfonato-calix[8]arene complex, H32 form
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