8ciw
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Methylsuccinyl-CoA dehydrogenase from Pseudomonas migulae with bound FAD and (2S)-methylsuccinyl-CoA== | |
| + | <StructureSection load='8ciw' size='340' side='right'caption='[[8ciw]], [[Resolution|resolution]] 1.93Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8ciw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_migulae Pseudomonas migulae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8CIW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8CIW FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.93Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=ZKK:(2S)-Methylsuccinyl-CoA'>ZKK</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ciw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ciw OCA], [https://pdbe.org/8ciw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ciw RCSB], [https://www.ebi.ac.uk/pdbsum/8ciw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ciw ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A1H5ILA2_9PSED A0A1H5ILA2_9PSED] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Nature has evolved eight different pathways for the capture and conversion of CO(2), including the Calvin-Benson-Bassham cycle of photosynthesis. Yet, these pathways underlie constrains and only represent a fraction of the thousands of theoretically possible solutions. To overcome the limitations of natural evolution, we introduce the HydrOxyPropionyl-CoA/Acrylyl-CoA (HOPAC) cycle, a new-to-nature CO(2)-fixation pathway that was designed through metabolic retrosynthesis around the reductive carboxylation of acrylyl-CoA, a highly efficient principle of CO(2) fixation. We realized the HOPAC cycle in a step-wise fashion and used rational engineering approaches and machine learning-guided workflows to further optimize its output by more than one order of magnitude. Version 4.0 of the HOPAC cycle encompasses 11 enzymes from six different organisms, converting ~3.0 mM CO(2) into glycolate within 2 hours. Our work moves the hypothetical HOPAC cycle from a theoretical design into an established in vitro system that forms the basis for different potential applications. | ||
| - | + | Exploring alternative pathways for the in vitro establishment of the HOPAC cycle for synthetic CO(2) fixation.,McLean R, Schwander T, Diehl C, Cortina NS, Paczia N, Zarzycki J, Erb TJ Sci Adv. 2023 Jun 16;9(24):eadh4299. doi: 10.1126/sciadv.adh4299. Epub 2023 Jun , 14. PMID:37315145<ref>PMID:37315145</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 8ciw" style="background-color:#fffaf0;"></div> |
| - | [[Category: Erb | + | == References == |
| - | [[Category: Zarzycki | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Pseudomonas migulae]] | ||
| + | [[Category: Erb TJ]] | ||
| + | [[Category: McLean R]] | ||
| + | [[Category: Zarzycki J]] | ||
Revision as of 07:18, 12 July 2023
Methylsuccinyl-CoA dehydrogenase from Pseudomonas migulae with bound FAD and (2S)-methylsuccinyl-CoA
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