8if6
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Conformational Dynamics of the D53-D3-D14 Complex in Strigolactone Signaling== | |
| + | <StructureSection load='8if6' size='340' side='right'caption='[[8if6]], [[Resolution|resolution]] 7.09Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8if6]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa_Japonica_Group Oryza sativa Japonica Group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8IF6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8IF6 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 7.09Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8if6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8if6 OCA], [https://pdbe.org/8if6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8if6 RCSB], [https://www.ebi.ac.uk/pdbsum/8if6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8if6 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/MAX2_ORYSJ MAX2_ORYSJ] Controls tillering by suppressing axillary bud activity. Tiller is a specialized grain-bearing branch that is formed on the unelongated basal internode and grows independently of the mother stem (culm) by means of its own adventitious roots. Is necessary for responses to strigolactones and the establishment of arbuscular mycorrhiza symbiosis in rice. May be involved in the ubiquitin-mediated degradation of specific proteins that activate axillary growth. D3 is crucial for establishing arbuscular mycorrhiza (AM) symbiosis in rice, whereas D14 and D14-LIKE are not. Our results suggest distinct roles for these SL-related components in AM symbiosis. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Strigolactones (SLs) play fundamental roles in regulating plant architecture, which is a major factor determining crop yield. The perception and signal transduction of SLs require the formation of a complex containing the receptor DWARF14 (D14), an F-box protein D3 and a transcriptional regulator D53 in an SL-dependent manner. Structural and biochemical analyses of D14 and its orthologues DAD2 and AtD14, D3, and the complexes of ASK1-D3-AtD14 and D3CTH-D14 have made great contributions to understanding the mechanisms of SL perception. However, structural analyses of D53 and the D53-D3-D14 holo-complex are challenging, and the biochemical mechanism underlying complex assembly remains poorly understood. Here, we found that apo-D53 was rather flexible and reconstituted the holo-complex containing D53, SKP1, D3, and D14 with rac-GR24. The cryo-EM structure of SKP1-D3-D14 in the presence of D53 was analyzed and superimposed on the crystal structure of ASK1-D3-AtD14 without D53. No large conformational rearrangement was observed but a 9 A rotation appeared between D14 and AtD14. Using hydrogen-deuterium exchange monitored by mass spectrometry, we analyzed dynamic motifs of D14, D3 and D53 in the D53-SKP1-D3-D14 complex assembly process and further identified two potential interfaces in D53 that are located in the N and D2 domains, respectively. Together, our results uncovered the dynamic conformational changes and built a model of the holo-complex D53-SKP1-D3-D14, offering valuable information for the biochemical and genetic mechanisms of SL perception and signal transduction. | ||
| - | + | Conformational Dynamics of the D53-D3-D14 Complex in Strigolactone Signaling.,Liu S, Wang J, Song B, Gong X, Liu H, Hu Q, Zhang J, Li Q, Zheng J, Wang H, Xu HE, Li J, Wang B Plant Cell Physiol. 2023 Jun 29:pcad067. doi: 10.1093/pcp/pcad067. PMID:37384578<ref>PMID:37384578</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Liu | + | <div class="pdbe-citations 8if6" style="background-color:#fffaf0;"></div> |
| - | [[Category: Wang | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Oryza sativa Japonica Group]] | ||
| + | [[Category: Liu SM]] | ||
| + | [[Category: Wang J]] | ||
Revision as of 07:26, 12 July 2023
Conformational Dynamics of the D53-D3-D14 Complex in Strigolactone Signaling
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