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| | <StructureSection load='5eq4' size='340' side='right'caption='[[5eq4]], [[Resolution|resolution]] 2.30Å' scene=''> | | <StructureSection load='5eq4' size='340' side='right'caption='[[5eq4]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5eq4]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Strsv Strsv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EQ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EQ4 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5eq4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_sanguinis_SK36 Streptococcus sanguinis SK36]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EQ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EQ4 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5eq2|5eq2]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">srpA, SSA_0829 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=388919 STRSV])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5eq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eq4 OCA], [https://pdbe.org/5eq4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5eq4 RCSB], [https://www.ebi.ac.uk/pdbsum/5eq4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5eq4 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5eq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eq4 OCA], [http://pdbe.org/5eq4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5eq4 RCSB], [http://www.ebi.ac.uk/pdbsum/5eq4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5eq4 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A3CM52_STRSV A3CM52_STRSV] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Strsv]] | + | [[Category: Streptococcus sanguinis SK36]] |
| - | [[Category: Anderson, S]] | + | [[Category: Anderson S]] |
| - | [[Category: Iverson, T M]] | + | [[Category: Iverson TM]] |
| - | [[Category: Loukachevitch, L V]] | + | [[Category: Loukachevitch LV]] |
| - | [[Category: McCulloch, K M]] | + | [[Category: McCulloch KM]] |
| - | [[Category: Vann, K R]] | + | [[Category: Vann KR]] |
| - | [[Category: Wawrzak, Z]] | + | [[Category: Wawrzak Z]] |
| - | [[Category: Bacterial adhesin]]
| + | |
| - | [[Category: Immunoglobulin fold]]
| + | |
| - | [[Category: Lectin]]
| + | |
| - | [[Category: Serine-rich repeat]]
| + | |
| - | [[Category: Sugar binding protein]]
| + | |
| Structural highlights
Function
A3CM52_STRSV
Publication Abstract from PubMed
Streptococcus sanguinis is a leading cause of infective endocarditis, a life-threatening infection of the cardiovascular system. An important interaction in the pathogenesis of infective endocarditis is attachment of the organisms to host platelets. S. sanguinis expresses a serine-rich repeat adhesin, SrpA, similar in sequence to platelet-binding adhesins associated with increased virulence in this disease. In this study, we determined the first crystal structure of the putative binding region of SrpA (SrpABR) both unliganded and in complex with a synthetic disaccharide ligand at 1.8 A and 2.0 A resolution, respectively. We identified a conserved sequence Thr-Arg motif that orients the sialic acid moiety and is required for binding to platelet monolayers. Further, we propose that sequence insertions in closely related family members contribute to the modulation of structural and functional properties, including the quaternary structure, the tertiary structure, and the ligand binding site.
Structural Basis for Sialoglycan Binding by the Streptococcus sanguinis SrpA Adhesin.,Bensing BA, Loukachevitch LV, McCulloch KM, Yu H, Vann KR, Wawrzak Z, Anderson S, Chen X, Sullam PM, Iverson TM J Biol Chem. 2016 Feb 1. pii: jbc.M115.701425. PMID:26833566[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Bensing BA, Loukachevitch LV, McCulloch KM, Yu H, Vann KR, Wawrzak Z, Anderson S, Chen X, Sullam PM, Iverson TM. Structural Basis for Sialoglycan Binding by the Streptococcus sanguinis SrpA Adhesin. J Biol Chem. 2016 Feb 1. pii: jbc.M115.701425. PMID:26833566 doi:http://dx.doi.org/10.1074/jbc.M115.701425
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