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| | <StructureSection load='5eqn' size='340' side='right'caption='[[5eqn]], [[Resolution|resolution]] 2.30Å' scene=''> | | <StructureSection load='5eqn' size='340' side='right'caption='[[5eqn]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5eqn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"streptomyces_rubellomurinus"_iguchi_et_al._1980 "streptomyces rubellomurinus" iguchi et al. 1980]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EQN OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5EQN FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5eqn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_rubellomurinus Streptomyces rubellomurinus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EQN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EQN FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">frbJ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=359131 "Streptomyces rubellomurinus" Iguchi et al. 1980])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5eqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eqn OCA], [https://pdbe.org/5eqn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5eqn RCSB], [https://www.ebi.ac.uk/pdbsum/5eqn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5eqn ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5eqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eqn OCA], [http://pdbe.org/5eqn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5eqn RCSB], [http://www.ebi.ac.uk/pdbsum/5eqn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5eqn ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q0ZQ39_STRR3 Q0ZQ39_STRR3] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Streptomyces rubellomurinus iguchi et al. 1980]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Hu, Y]] | + | [[Category: Streptomyces rubellomurinus]] |
| - | [[Category: Li, C]] | + | [[Category: Hu Y]] |
| - | [[Category: Zhang, H]] | + | [[Category: Li C]] |
| - | [[Category: Dioxygenase]] | + | [[Category: Zhang H]] |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Phosphonate hydroxylase]]
| + | |
| Structural highlights
Function
Q0ZQ39_STRR3
Publication Abstract from PubMed
FrbJ is a member of the Fe(2+)/alpha-ketoglutarate-dependent dioxygenase family which hydroxylates the natural product FR-900098 of Streptomyces rubellomurinus, yielding the phosphonate antibiotic FR-33289. Here, the crystal structure of FrbJ, which shows structural homology to taurine dioxygenase (TauD), a key member of the same family, is reported. Unlike other members of the family, FrbJ has an unusual lid structure which consists of two beta-strands with a long loop between them. To investigate the role of this lid motif, a molecular-dynamics simulation was performed with the FrbJ structure. The molecular-dynamics simulation analysis implies that the lid-loop region is highly flexible, which is consistent with the fact that FrbJ has a relatively broad spectrum of substrates with different lengths. Interestingly, an access tunnel is found at the back of the active site which connects the putative binding site of alpha-ketoglutarate to the solvent outside.
Structural analysis of a phosphonate hydroxylase with an access tunnel at the back of the active site.,Li C, Junaid M, Almuqri EA, Hao S, Zhang H Acta Crystallogr F Struct Biol Commun. 2016 May 1;72(Pt 5):362-8. doi:, 10.1107/S2053230X16004933. Epub 2016 Apr 22. PMID:27139827[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Li C, Junaid M, Almuqri EA, Hao S, Zhang H. Structural analysis of a phosphonate hydroxylase with an access tunnel at the back of the active site. Acta Crystallogr F Struct Biol Commun. 2016 May 1;72(Pt 5):362-8. doi:, 10.1107/S2053230X16004933. Epub 2016 Apr 22. PMID:27139827 doi:http://dx.doi.org/10.1107/S2053230X16004933
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