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| | <StructureSection load='5ey6' size='340' side='right'caption='[[5ey6]], [[Resolution|resolution]] 1.90Å' scene=''> | | <StructureSection load='5ey6' size='340' side='right'caption='[[5ey6]], [[Resolution|resolution]] 1.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5ey6]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EY6 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5EY6 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5ey6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Populus_trichocarpa Populus trichocarpa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EY6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EY6 FirstGlance]. <br> |
| - | </td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5ey6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ey6 OCA], [http://pdbe.org/5ey6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ey6 RCSB], [http://www.ebi.ac.uk/pdbsum/5ey6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ey6 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ey6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ey6 OCA], [https://pdbe.org/5ey6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ey6 RCSB], [https://www.ebi.ac.uk/pdbsum/5ey6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ey6 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/B9GQ64_POPTR B9GQ64_POPTR] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Glutathione transferase]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Didierjean, C]] | + | [[Category: Populus trichocarpa]] |
| - | [[Category: Gense, F]] | + | [[Category: Didierjean C]] |
| - | [[Category: Pegeot, H]] | + | [[Category: Gense F]] |
| - | [[Category: Rouhier, N]] | + | [[Category: Pegeot H]] |
| - | [[Category: Glutathione]]
| + | [[Category: Rouhier N]] |
| - | [[Category: Ligandin]]
| + | |
| - | [[Category: Transferase]]
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| Structural highlights
Function
B9GQ64_POPTR
Publication Abstract from PubMed
The glutathione transferase (GST) gene family is divided into 14 classes in photosynthetic organisms. Among them, the Phi class (GSTF) is composed of a large number of genes that are often induced in response to environmental constraints due to their ability to detoxify xenobiotics, to their peroxidase activity and to their involvement in the biosynthesis and/or transport of secondary metabolites. However, the exact functions of GSTFs from many plants including Populus trichocarpa are unknown. Here, following GSTF1 characterization, we have performed a comparative analysis of the seven other GSTFs found in poplar by systematically evaluating the biochemical and enzymatic properties of the corresponding recombinant proteins and of variants mutated for active site residues and by determining the three-dimensional structures of several representatives. Owing to the presence of a cysteine with a pKa value around 5 in their active site, GSTF3, F7, and F8 displayed a thiol transferase activity in addition to the usual glutathione transferase and peroxidase activities. From structural analyses, it appeared that these dual biochemical properties originate from the existence of a certain variability in the beta1-alpha1 loop. This allows positioning of several active site residues at proximity of the glutathione molecule, which itself remains unchanged in GSTF three-dimensional structures. These results highlight the promiscuity of some GSTFs and that changes of active site residues in some isoforms during evolution generated functional diversity by modifying their activity profile. DATABASE: Structural data are available in the PDB under the accession numbers 5EY6, 5F05, 5F06, and 5F07.
Structural plasticity among glutathione transferase Phi members: natural combination of catalytic residues confers dual biochemical activities.,Pegeot H, Mathiot S, Perrot T, Gense F, Hecker A, Didierjean C, Rouhier N FEBS J. 2017 Jun 16. doi: 10.1111/febs.14138. PMID:28622459[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Pegeot H, Mathiot S, Perrot T, Gense F, Hecker A, Didierjean C, Rouhier N. Structural plasticity among glutathione transferase Phi members: natural combination of catalytic residues confers dual biochemical activities. FEBS J. 2017 Jun 16. doi: 10.1111/febs.14138. PMID:28622459 doi:http://dx.doi.org/10.1111/febs.14138
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