1lju

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[[Image:1lju.jpg|left|200px]]
[[Image:1lju.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1lju |SIZE=350|CAPTION= <scene name='initialview01'>1lju</scene>, resolution 1.4&Aring;
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The line below this paragraph, containing "STRUCTURE_1lju", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CSR:S-ARSONOCYSTEINE'>CSR</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Arsenate_reductase_(glutaredoxin) Arsenate reductase (glutaredoxin)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.20.4.1 1.20.4.1] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= arsc ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 Staphylococcus aureus])
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-->
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|DOMAIN=
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{{STRUCTURE_1lju| PDB=1lju | SCENE= }}
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|RELATEDENTRY=[[1jf8|1JF8]], [[1ljl|1LJL]], [[1lk0|1LK0]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lju FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lju OCA], [http://www.ebi.ac.uk/pdbsum/1lju PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1lju RCSB]</span>
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}}
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'''X-RAY STRUCTURE OF C15A ARSENATE REDUCTASE FROM PI258 COMPLEXED WITH ARSENITE'''
'''X-RAY STRUCTURE OF C15A ARSENATE REDUCTASE FROM PI258 COMPLEXED WITH ARSENITE'''
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==Reference==
==Reference==
All intermediates of the arsenate reductase mechanism, including an intramolecular dynamic disulfide cascade., Messens J, Martins JC, Van Belle K, Brosens E, Desmyter A, De Gieter M, Wieruszeski JM, Willem R, Wyns L, Zegers I, Proc Natl Acad Sci U S A. 2002 Jun 25;99(13):8506-11. Epub 2002 Jun 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12072565 12072565]
All intermediates of the arsenate reductase mechanism, including an intramolecular dynamic disulfide cascade., Messens J, Martins JC, Van Belle K, Brosens E, Desmyter A, De Gieter M, Wieruszeski JM, Willem R, Wyns L, Zegers I, Proc Natl Acad Sci U S A. 2002 Jun 25;99(13):8506-11. Epub 2002 Jun 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12072565 12072565]
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[[Category: Arsenate reductase (glutaredoxin)]]
 
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
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[[Category: Wyns, L.]]
[[Category: Wyns, L.]]
[[Category: Zegers, I.]]
[[Category: Zegers, I.]]
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[[Category: p-loop]]
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[[Category: P-loop]]
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[[Category: ptpase i fold]]
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[[Category: Ptpase i fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:59:13 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:03:37 2008''
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Revision as of 20:59, 2 May 2008

Image:1lju.jpg

Template:STRUCTURE 1lju

X-RAY STRUCTURE OF C15A ARSENATE REDUCTASE FROM PI258 COMPLEXED WITH ARSENITE


Overview

The mechanism of pI258 arsenate reductase (ArsC) catalyzed arsenate reduction, involving its P-loop structural motif and three redox active cysteines, has been unraveled. All essential intermediates are visualized with x-ray crystallography, and NMR is used to map dynamic regions in a key disulfide intermediate. Steady-state kinetics of ArsC mutants gives a view of the crucial residues for catalysis. ArsC combines a phosphatase-like nucleophilic displacement reaction with a unique intramolecular disulfide bond cascade. Within this cascade, the formation of a disulfide bond triggers a reversible "conformational switch" that transfers the oxidative equivalents to the surface of the protein, while releasing the reduced substrate.

About this Structure

1LJU is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.

Reference

All intermediates of the arsenate reductase mechanism, including an intramolecular dynamic disulfide cascade., Messens J, Martins JC, Van Belle K, Brosens E, Desmyter A, De Gieter M, Wieruszeski JM, Willem R, Wyns L, Zegers I, Proc Natl Acad Sci U S A. 2002 Jun 25;99(13):8506-11. Epub 2002 Jun 18. PMID:12072565 Page seeded by OCA on Fri May 2 23:59:13 2008

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