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| | <StructureSection load='5ez7' size='340' side='right'caption='[[5ez7]], [[Resolution|resolution]] 2.40Å' scene=''> | | <StructureSection load='5ez7' size='340' side='right'caption='[[5ez7]], [[Resolution|resolution]] 2.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5ez7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EZ7 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5EZ7 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5ez7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EZ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EZ7 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PA4991 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5ez7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ez7 OCA], [http://pdbe.org/5ez7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ez7 RCSB], [http://www.ebi.ac.uk/pdbsum/5ez7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ez7 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ez7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ez7 OCA], [https://pdbe.org/5ez7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ez7 RCSB], [https://www.ebi.ac.uk/pdbsum/5ez7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ez7 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Y4991_PSEAE Y4991_PSEAE] Probably functions as a FAD-dependent oxidoreductase, whose physiological substrate is unknown. Does not display amino-acid oxidase or glycerol-3-phosphate dehydrogenase activities (PubMed:26841760). Is essential for growth of P.aeruginosa in the sputum of cystic fibrosis patients (PubMed:25775563).<ref>PMID:25775563</ref> <ref>PMID:26841760</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Pseae]] | + | [[Category: Pseudomonas aeruginosa PAO1]] |
| - | [[Category: Jacewicz, A]] | + | [[Category: Jacewicz A]] |
| - | [[Category: Lindqvist, Y]] | + | [[Category: Lindqvist Y]] |
| - | [[Category: Schneider, G]] | + | [[Category: Schneider G]] |
| - | [[Category: Schnell, R]] | + | [[Category: Schnell R]] |
| - | [[Category: Flavine]]
| + | |
| - | [[Category: Flavoenzyme]]
| + | |
| - | [[Category: Oxidoreductase]]
| + | |
| Structural highlights
Function
Y4991_PSEAE Probably functions as a FAD-dependent oxidoreductase, whose physiological substrate is unknown. Does not display amino-acid oxidase or glycerol-3-phosphate dehydrogenase activities (PubMed:26841760). Is essential for growth of P.aeruginosa in the sputum of cystic fibrosis patients (PubMed:25775563).[1] [2]
Publication Abstract from PubMed
The locus PA4991 in Pseudomonas aeruginosa encodes an open reading frame that has been identified as essential for the virulence and/or survival of this pathogenic organism in the infected host. Here, it is shown that this gene encodes a monomeric FAD-binding protein of molecular mass 42.2 kDa. The structure of PA4991 was determined by a combination of molecular replacement using a search model generated with Rosetta and phase improvement by a low-occupancy heavy-metal derivative. PA4991 belongs to the GR2 family of FAD-dependent oxidoreductases, comprising an FAD-binding domain typical of the glutathione reductase family and a second domain dominated by an eight-stranded mixed beta-sheet. Most of the protein-FAD interactions are via the FAD-binding domain, but the isoalloxazine ring is located at the domain interface and interacts with residues from both domains. A comparison with the structurally related glycine oxidase and glycerol-3-phosphate dehydrogenase shows that in spite of very low amino-acid sequence identity (<18%) several active-site residues involved in substrate binding in these enzymes are conserved in PA4991. However, enzymatic assays show that PA4991 does not display amino-acid oxidase or glycerol-3-phosphate dehydrogenase activities, suggesting that it requires different substrates for activity.
Crystal structure of the flavoenzyme PA4991 from Pseudomonas aeruginosa.,Jacewicz A, Schnell R, Lindqvist Y, Schneider G Acta Crystallogr F Struct Biol Commun. 2016 Feb 1;72(Pt 2):105-11. doi:, 10.1107/S2053230X15024437. Epub 2016 Jan 22. PMID:26841760[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Turner KH, Wessel AK, Palmer GC, Murray JL, Whiteley M. Essential genome of Pseudomonas aeruginosa in cystic fibrosis sputum. Proc Natl Acad Sci U S A. 2015 Mar 31;112(13):4110-5. PMID:25775563 doi:10.1073/pnas.1419677112
- ↑ Jacewicz A, Schnell R, Lindqvist Y, Schneider G. Crystal structure of the flavoenzyme PA4991 from Pseudomonas aeruginosa. Acta Crystallogr F Struct Biol Commun. 2016 Feb 1;72(Pt 2):105-11. doi:, 10.1107/S2053230X15024437. Epub 2016 Jan 22. PMID:26841760 doi:http://dx.doi.org/10.1107/S2053230X15024437
- ↑ Jacewicz A, Schnell R, Lindqvist Y, Schneider G. Crystal structure of the flavoenzyme PA4991 from Pseudomonas aeruginosa. Acta Crystallogr F Struct Biol Commun. 2016 Feb 1;72(Pt 2):105-11. doi:, 10.1107/S2053230X15024437. Epub 2016 Jan 22. PMID:26841760 doi:http://dx.doi.org/10.1107/S2053230X15024437
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