1lck

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spinqualitylabelsall models 1lck, resolution 2.5Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

SH3-SH2 DOMAIN FRAGMENT OF HUMAN P56-LCK TYROSINE KINASE COMPLEXED WITH THE 10 RESIDUE SYNTHETIC PHOSPHOTYROSYL PEPTIDE TEGQPYQPQPA

Contents 1 Overview 2 Disease 3 About this Structure 4 Reference

Overview

The kinase p56lck (Lck) is a T-lymphocyte-specific member of the Src, family of non-receptor tyrosine kinases. Members of the Src family each, contain unique amino-terminal regions, followed by Src-homology domains, SH3 and SH2, and a tyrosine kinase domain. SH3 and SH2 domains mediate, critical protein interactions in many signal-transducing pathways. They, are small, independently folded modules of about 60 and 100 residues, respectively, and they are often but not always found together in the same, molecule. Like all nine Src-family kinases (reviewed in ref. 3), Lck is, regulated by phosphorylation of a tyrosine in the short C-terminal tail of, its catalytic domain. There is evidence that binding of the phosphorylated, tail to the SH2 domain inhibits catalytic activity of the kinase domain, and that the SH3 and SH2 domains may act together to effect this, regulation. Here we report the crystal structures for a fragment of Lck, bearing its SH3 and SH2 domains, alone and in complex with a, phosphotyrosyl peptide containing the sequence of the Lck C-terminal, regulatory tail. The latter complex represents the regulatory apparatus of, Lck. The SH3-SH2 fragment forms similar dimers in both crystals, and the, tail peptide binds at the intermolecular SH3/SH2 contact. The two, structures show how an SH3 domain might recognize a specific target and, suggest how dimerization could play a role in regulating Src-family, kinases.

Disease

Known disease associated with this structure: SCID due to LCK deficiency OMIM:[153390]

About this Structure

1LCK is a Protein complex structure of sequences from Homo sapiens with PHS as ligand. Active as Transferase, with EC number and 2.7.10.2 2.7.10.1 and 2.7.10.2 Full crystallographic information is available from OCA.

Reference

Structure of the regulatory domains of the Src-family tyrosine kinase Lck., Eck MJ, Atwell SK, Shoelson SE, Harrison SC, Nature. 1994 Apr 21;368(6473):764-9. PMID:7512222

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