1llf

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[[Image:1llf.gif|left|200px]]
[[Image:1llf.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1llf |SIZE=350|CAPTION= <scene name='initialview01'>1llf</scene>, resolution 1.4&Aring;
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The line below this paragraph, containing "STRUCTURE_1llf", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=F23:TRICOSANOIC+ACID'>F23</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1llf| PDB=1llf | SCENE= }}
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|RELATEDENTRY=[[1cle|1CLE]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1llf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1llf OCA], [http://www.ebi.ac.uk/pdbsum/1llf PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1llf RCSB]</span>
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}}
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'''Cholesterol Esterase (Candida Cylindracea) Crystal Structure at 1.4A resolution'''
'''Cholesterol Esterase (Candida Cylindracea) Crystal Structure at 1.4A resolution'''
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[[Category: Pletnev, V.]]
[[Category: Pletnev, V.]]
[[Category: Wawrzak, Z.]]
[[Category: Wawrzak, Z.]]
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[[Category: candida cylindracea cholesterol esterase]]
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[[Category: Candida cylindracea cholesterol esterase]]
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[[Category: crystal structure]]
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[[Category: Crystal structure]]
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[[Category: sterol ester acylhydrolase]]
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[[Category: Sterol ester acylhydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:02:06 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:04:16 2008''
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Revision as of 21:02, 2 May 2008

Image:1llf.gif

Template:STRUCTURE 1llf

Cholesterol Esterase (Candida Cylindracea) Crystal Structure at 1.4A resolution


Overview

The three-dimensional structure of a Candida cylindracea cholesterol esterase (ChE) homodimer (534 x 2 amino acids) in complex with a ligand of proposed formula C(23)H(48)O(2) has been determined at 1.4 A resolution in space group P1 using synchrotron low-temperature data. The structure refined to R = 0.136 and R(free) = 0.169 and has revealed new stereochemical details in addition to those detected for the apo- and holo-forms at 1.9 and 2.0 A resolution, respectively [Ghosh et al. (1995), Structure, 3, 279-288]. The cholesterol esterase structure is a dimer with four spatially separated interfacial contact areas and two symmetry-related pairs of openings to an internal intradimer cavity. Hydrophobic active-site gorges in each subunit face each other across a central interfacial cavity. The ChE subunits have carbohydrate chains attached to their Asn314 and Asn351 residues, with two ordered N-acetyl-D-glucosoamine moieties visible at each site. The side chains of 14 residues have two alternative conformations with occupancy values of 0.5 +/- 0.2. For each subunit the electron density in the enzyme active-site gorge is well modeled by a C(23)-chain fatty acid.

About this Structure

1LLF is a Single protein structure of sequence from Candida cylindracea. Full crystallographic information is available from OCA.

Reference

Three-dimensional structure of homodimeric cholesterol esterase-ligand complex at 1.4 A resolution., Pletnev V, Addlagatta A, Wawrzak Z, Duax W, Acta Crystallogr D Biol Crystallogr. 2003 Jan;59(Pt 1):50-6. Epub 2002 Dec, 19. PMID:12499539 Page seeded by OCA on Sat May 3 00:02:06 2008

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