5f8b

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==Crystallographic Structure of PsoE with Co==
==Crystallographic Structure of PsoE with Co==
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<StructureSection load='5f8b' size='340' side='right' caption='[[5f8b]], [[Resolution|resolution]] 2.54&Aring;' scene=''>
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<StructureSection load='5f8b' size='340' side='right'caption='[[5f8b]], [[Resolution|resolution]] 2.54&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5f8b]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspfu Aspfu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F8B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5F8B FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5f8b]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_fumigatus_Af293 Aspergillus fumigatus Af293]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F8B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5F8B FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene>, <scene name='pdbligand=PZS:(5~{S},8~{S},9~{R})-2-[(~{E})-HEX-1-ENYL]-8-METHOXY-3-METHYL-9-OXIDANYL-8-(PHENYLCARBONYL)-1-OXA-7-AZASPIRO[4.4]NON-2-ENE-4,6-DIONE'>PZS</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.54&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AFUA_8G00580 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=330879 ASPFU])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene>, <scene name='pdbligand=PZS:(5~{S},8~{S},9~{R})-2-[(~{E})-HEX-1-ENYL]-8-METHOXY-3-METHYL-9-OXIDANYL-8-(PHENYLCARBONYL)-1-OXA-7-AZASPIRO[4.4]NON-2-ENE-4,6-DIONE'>PZS</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5f8b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f8b OCA], [http://pdbe.org/5f8b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5f8b RCSB], [http://www.ebi.ac.uk/pdbsum/5f8b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5f8b ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5f8b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f8b OCA], [https://pdbe.org/5f8b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5f8b RCSB], [https://www.ebi.ac.uk/pdbsum/5f8b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5f8b ProSAT]</span></td></tr>
</table>
</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PSOE_ASPFU PSOE_ASPFU] Glutathione S-transferase; part of the gene cluster that mediates the biosynthesis of pseurotin A, a competitive inhibitor of chitin synthase and an inducer of nerve-cell proliferation (PubMed:24082142, PubMed:24939566). The PKS-NRPS hybrid synthetase psoA is responsible for the biosynthesis of azaspirene, one of the first intermediates having the 1-oxa-7-azaspiro[4,4]-non-2-ene-4,6-dione core of pseurotin, via condensation of one acetyl-CoA, 4 malonyl-CoA, and a L-phenylalanine molecule (PubMed:24082142, PubMed:24939566). The dual-functional monooxygenase/methyltransferase psoF seems to be involved in the addition of the C3 methyl group onto the pseurotin scaffold (PubMed:24939566). Azaspirene is then converted to synerazol through 4 steps including oxidation of C17 by the cytochrome P450 monooxygenase psoD, O-methylation of the hydroxy group of C8 by the methyltransferase psoC, and the trans-to-cis isomerization of the C13 olefin by the glutathione S-transferase psoE (PubMed:24939566, PubMed:27072782). The fourth step of synerazol production is performed by the dual-functional monooxygenase/methyltransferase psoF which seems to catalyze the epoxidation of the intermediate deepoxy-synerazol (PubMed:24939566). Synerazol can be attacked by a water molecule nonenzymatically at two different positions to yield two diol products, pseurotin A and pseurotin D (PubMed:24939566).<ref>PMID:24082142</ref> <ref>PMID:24939566</ref> <ref>PMID:27072782</ref>
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Aspfu]]
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[[Category: Aspergillus fumigatus Af293]]
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[[Category: Hara, K]]
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[[Category: Large Structures]]
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[[Category: Hashimoto, H]]
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[[Category: Hara K]]
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[[Category: Tsunematsu, Y]]
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[[Category: Hashimoto H]]
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[[Category: Watanabe, K]]
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[[Category: Tsunematsu Y]]
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[[Category: Yamamoto, T]]
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[[Category: Watanabe K]]
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[[Category: Gst]]
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[[Category: Yamamoto T]]
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[[Category: Transferase]]
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Revision as of 06:41, 19 July 2023

Crystallographic Structure of PsoE with Co

PDB ID 5f8b

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