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| | <StructureSection load='5fc9' size='340' side='right'caption='[[5fc9]], [[Resolution|resolution]] 1.60Å' scene=''> | | <StructureSection load='5fc9' size='340' side='right'caption='[[5fc9]], [[Resolution|resolution]] 1.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5fc9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Candidatus_nitrosopumilus_maritimus_scm1 Candidatus nitrosopumilus maritimus scm1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FC9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FC9 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5fc9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Nitrosopumilus_maritimus_SCM1 Nitrosopumilus maritimus SCM1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FC9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FC9 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Nmar_1307 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=436308 Candidatus Nitrosopumilus maritimus SCM1])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fc9 OCA], [http://pdbe.org/5fc9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fc9 RCSB], [http://www.ebi.ac.uk/pdbsum/5fc9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fc9 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fc9 OCA], [https://pdbe.org/5fc9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fc9 RCSB], [https://www.ebi.ac.uk/pdbsum/5fc9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fc9 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A9A2G4_NITMS A9A2G4_NITMS] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Candidatus nitrosopumilus maritimus scm1]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Gao, Y G]] | + | [[Category: Nitrosopumilus maritimus SCM1]] |
| - | [[Category: Hosseinzadeh, P]] | + | [[Category: Gao Y-G]] |
| - | [[Category: Lu, Y]] | + | [[Category: Hosseinzadeh P]] |
| - | [[Category: Robinson, H]] | + | [[Category: Lu Y]] |
| - | [[Category: Cupredoxin]]
| + | [[Category: Robinson H]] |
| - | [[Category: Metal binding protein]]
| + | |
| - | [[Category: Nitrogen cycle]]
| + | |
| - | [[Category: No oxidation]]
| + | |
| - | [[Category: Open type 1 copper site]]
| + | |
| Structural highlights
Function
A9A2G4_NITMS
Publication Abstract from PubMed
Mononuclear cupredoxin proteins usually contain a coordinately saturated type 1 copper (T1Cu) center and function exclusively as electron carriers. Here we report a cupredoxin isolated from the nitrifying archaeon Nitrosopumilus maritimus SCM1, called Nmar1307, that contains a T1Cu center with an open binding site containing water. It displays a deep purple color due to strong absorptions around 413 nm (1880 M-1 cm-1) and 558 nm (2290 M-1 cm-1) in the UV-vis electronic spectrum. EPR studies suggest the protein contains two Cu(II) species of nearly equal population, one nearly axial, with hyperfine constant A parallel = 98 x 10-4 cm-1, and another more rhombic, with a smaller A parallel value of 69 x 10-4 cm-1. The X-ray crystal structure at 1.6 A resolution confirms that it contains a Cu atom coordinated by two His and one Cys in a trigonal plane, with an axial H2O at 2.25 A. Both UV-vis absorption and EPR spectroscopic studies suggest that the Nmar1307 can oxidize NO to nitrite, an activity that is attributable to the high reduction potential (354 mV vs SHE) of the copper site. These results suggest that mononuclear cupredoxins can have a wide range of structural features, including an open binding site containing water, making this class of proteins even more versatile.
A Purple Cupredoxin from Nitrosopumilus maritimus Containing a Mononuclear Type 1 Copper Center with an Open Binding Site.,Hosseinzadeh P, Tian S, Marshall NM, Hemp J, Mullen T, Nilges MJ, Gao YG, Robinson H, Stahl DA, Gennis RB, Lu Y J Am Chem Soc. 2016 May 12. PMID:27120678[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Hosseinzadeh P, Tian S, Marshall NM, Hemp J, Mullen T, Nilges MJ, Gao YG, Robinson H, Stahl DA, Gennis RB, Lu Y. A Purple Cupredoxin from Nitrosopumilus maritimus Containing a Mononuclear Type 1 Copper Center with an Open Binding Site. J Am Chem Soc. 2016 May 12. PMID:27120678 doi:http://dx.doi.org/10.1021/jacs.5b13128
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