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| | <SX load='5fj7' size='340' side='right' viewer='molstar' caption='[[5fj7]], [[Resolution|resolution]] 7.90Å' scene=''> | | <SX load='5fj7' size='340' side='right' viewer='molstar' caption='[[5fj7]], [[Resolution|resolution]] 7.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5fj7]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpph6 Bpph6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FJ7 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5FJ7 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5fj7]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_virus_phi6 Pseudomonas virus phi6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FJ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FJ7 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 7.9Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5fj5|5fj5]], [[5fj6|5fj6]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5fj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fj7 OCA], [http://pdbe.org/5fj7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fj7 RCSB], [http://www.ebi.ac.uk/pdbsum/5fj7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fj7 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fj7 OCA], [https://pdbe.org/5fj7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fj7 RCSB], [https://www.ebi.ac.uk/pdbsum/5fj7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fj7 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/P1_BPPH6 P1_BPPH6]] P1 is the major inner capsid (core) protein of the polyhedral procapsid, which is responsible for genomic replication and transcription. Forms a dodecahedral shell from 60 asymmetric dimers. Binds to RNA and may be involved in genomic packaging. | + | [https://www.uniprot.org/uniprot/P1_BPPH6 P1_BPPH6] P1 is the major inner capsid (core) protein of the polyhedral procapsid, which is responsible for genomic replication and transcription. Forms a dodecahedral shell from 60 asymmetric dimers. Binds to RNA and may be involved in genomic packaging. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </SX> | | </SX> |
| - | [[Category: Bpph6]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Huiskonen, J T]] | + | [[Category: Pseudomonas virus phi6]] |
| - | [[Category: Ilca, S]] | + | [[Category: Huiskonen JT]] |
| - | [[Category: Kotecha, A]] | + | [[Category: Ilca S]] |
| - | [[Category: Poranen, M P]] | + | [[Category: Kotecha A]] |
| - | [[Category: Stuart, D I]] | + | [[Category: Poranen MP]] |
| - | [[Category: Sun, X]] | + | [[Category: Stuart DI]] |
| - | [[Category: Bacteriophage phi6]]
| + | [[Category: Sun X]] |
| - | [[Category: P1]]
| + | |
| - | [[Category: P2]]
| + | |
| - | [[Category: Polymerase]]
| + | |
| - | [[Category: Polymerase complex]]
| + | |
| - | [[Category: Viral protein]]
| + | |
| Structural highlights
Function
P1_BPPH6 P1 is the major inner capsid (core) protein of the polyhedral procapsid, which is responsible for genomic replication and transcription. Forms a dodecahedral shell from 60 asymmetric dimers. Binds to RNA and may be involved in genomic packaging.
Publication Abstract from PubMed
Electron cryomicroscopy can yield near-atomic resolution structures of highly ordered macromolecular complexes. Often however some subunits bind in a flexible manner, have different symmetry from the rest of the complex, or are present in sub-stoichiometric amounts, limiting the attainable resolution. Here we report a general method for the localized three-dimensional reconstruction of such subunits. After determining the particle orientations, local areas corresponding to the subunits can be extracted and treated as single particles. We demonstrate the method using three examples including a flexible assembly and complexes harbouring subunits with either partial occupancy or mismatched symmetry. Most notably, the method allows accurate fitting of the monomeric RNA-dependent RNA polymerase bound at the threefold axis of symmetry inside a viral capsid, revealing for the first time its exact orientation and interactions with the capsid proteins. Localized reconstruction is expected to provide novel biological insights in a range of challenging biological systems.
Localized reconstruction of subunits from electron cryomicroscopy images of macromolecular complexes.,Ilca SL, Kotecha A, Sun X, Poranen MM, Stuart DI, Huiskonen JT Nat Commun. 2015 Nov 4;6:8843. doi: 10.1038/ncomms9843. PMID:26534841[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Ilca SL, Kotecha A, Sun X, Poranen MM, Stuart DI, Huiskonen JT. Localized reconstruction of subunits from electron cryomicroscopy images of macromolecular complexes. Nat Commun. 2015 Nov 4;6:8843. doi: 10.1038/ncomms9843. PMID:26534841 doi:http://dx.doi.org/10.1038/ncomms9843
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