This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


5fkv

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (06:55, 19 July 2023) (edit) (undo)
 
Line 3: Line 3:
<SX load='5fkv' size='340' side='right' viewer='molstar' caption='[[5fkv]], [[Resolution|resolution]] 8.00&Aring;' scene=''>
<SX load='5fkv' size='340' side='right' viewer='molstar' caption='[[5fkv]], [[Resolution|resolution]] 8.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[5fkv]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FKV OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5FKV FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[5fkv]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FKV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FKV FirstGlance]. <br>
-
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5fku|5fku]], [[5fkw|5fkw]]</td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 8&#8491;</td></tr>
-
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fkv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fkv OCA], [https://pdbe.org/5fkv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fkv RCSB], [https://www.ebi.ac.uk/pdbsum/5fkv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fkv ProSAT]</span></td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5fkv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fkv OCA], [http://pdbe.org/5fkv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fkv RCSB], [http://www.ebi.ac.uk/pdbsum/5fkv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fkv ProSAT]</span></td></tr>
+
</table>
</table>
== Function ==
== Function ==
-
[[http://www.uniprot.org/uniprot/DPO3X_ECOLI DPO3X_ECOLI]] DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. Isoform tau: serves as a scaffold to help in the dimerization of the core complex. Isoform gamma: seems to interact with the delta subunit. to transfer the beta subunit on the DNA. [[http://www.uniprot.org/uniprot/DPO3A_ECOLI DPO3A_ECOLI]] DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase. [[http://www.uniprot.org/uniprot/DPO3E_ECOLI DPO3E_ECOLI]] DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. The epsilon subunit contain the editing function and is a proofreading 3'-5' exonuclease. [[http://www.uniprot.org/uniprot/DPO3B_ECOLI DPO3B_ECOLI]] DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped onto DNA, it slides freely (bidirectional and ATP-independent) along duplex DNA.
+
[https://www.uniprot.org/uniprot/DPO3A_ECOLI DPO3A_ECOLI] DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase.
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
Line 26: Line 25:
__TOC__
__TOC__
</SX>
</SX>
-
[[Category: DNA-directed DNA polymerase]]
+
[[Category: Escherichia coli K-12]]
-
[[Category: Ecoli]]
+
[[Category: Large Structures]]
[[Category: Large Structures]]
-
[[Category: Conrad, J]]
+
[[Category: Synthetic construct]]
-
[[Category: Fernandez-Leiro, R]]
+
[[Category: Conrad J]]
-
[[Category: Lamers, M H]]
+
[[Category: Fernandez-Leiro R]]
-
[[Category: Scheres, S H.W]]
+
[[Category: Lamers MH]]
-
[[Category: Dna polymerase iii alpha]]
+
[[Category: Scheres SHW]]
-
[[Category: Dna polymerase iii beta]]
+
-
[[Category: Dna polymerase iii epsilon]]
+
-
[[Category: Dna polymerase iii tau]]
+
-
[[Category: Dna replication]]
+
-
[[Category: Transferase]]
+

Current revision

cryo-EM structure of the E. coli replicative DNA polymerase complex bound to DNA (DNA polymerase III alpha, beta, epsilon, tau complex)

5fkv, resolution 8.00Å

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5fkv"
Personal tools