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| | <SX load='5fna' size='340' side='right' viewer='molstar' caption='[[5fna]], [[Resolution|resolution]] 4.80Å' scene=''> | | <SX load='5fna' size='340' side='right' viewer='molstar' caption='[[5fna]], [[Resolution|resolution]] 4.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5fna]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FNA OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5FNA FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5fna]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FNA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FNA FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CASP1, IL1BC, IL1BCE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.8Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Caspase-1 Caspase-1], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.36 3.4.22.36] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fna FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fna OCA], [https://pdbe.org/5fna PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fna RCSB], [https://www.ebi.ac.uk/pdbsum/5fna PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fna ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5fna FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fna OCA], [http://pdbe.org/5fna PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fna RCSB], [http://www.ebi.ac.uk/pdbsum/5fna PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fna ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CASP1_HUMAN CASP1_HUMAN]] Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.<ref>PMID:7876192</ref> <ref>PMID:15498465</ref> | + | [https://www.uniprot.org/uniprot/CASP1_HUMAN CASP1_HUMAN] Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.<ref>PMID:7876192</ref> <ref>PMID:15498465</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </SX> | | </SX> |
| - | [[Category: Caspase-1]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Human]]
| + | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Chen, S]] | + | [[Category: Chen S]] |
| - | [[Category: Fu, T M]] | + | [[Category: Fu TM]] |
| - | [[Category: Li, Y]] | + | [[Category: Li Y]] |
| - | [[Category: Lu, A]] | + | [[Category: Lu A]] |
| - | [[Category: Mao, Y]] | + | [[Category: Mao Y]] |
| - | [[Category: Ploegh, H L]] | + | [[Category: Ploegh HL]] |
| - | [[Category: Schmidt, F I]] | + | [[Category: Schmidt FI]] |
| - | [[Category: Tong, A B]] | + | [[Category: Tong AB]] |
| - | [[Category: Wu, H]] | + | [[Category: Wu H]] |
| - | [[Category: Yin, Q]] | + | [[Category: Yin Q]] |
| - | [[Category: Card]]
| + | |
| - | [[Category: Death domain]]
| + | |
| - | [[Category: Filament]]
| + | |
| - | [[Category: Helical reconstruction]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Inflammasome]]
| + | |
| - | [[Category: Signalosome]]
| + | |
| Structural highlights
Function
CASP1_HUMAN Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.[1] [2]
Publication Abstract from PubMed
Inflammasomes are cytosolic caspase-1-activation complexes that sense intrinsic and extrinsic danger signals, and trigger inflammatory responses and pyroptotic cell death. Homotypic interactions among Pyrin domains and caspase recruitment domains (CARDs) in inflammasome-complex components mediate oligomerization into filamentous assemblies. Several cytosolic proteins consisting of only interaction domains exert inhibitory effects on inflammasome assembly. In this study, we determined the structure of the human caspase-1 CARD domain (caspase-1(CARD)) filament by cryo-electron microscopy and investigated the biophysical properties of two caspase-1-like CARD-only proteins: human inhibitor of CARD (INCA or CARD17) and ICEBERG (CARD18). Our results reveal that INCA caps caspase-1 filaments, thereby exerting potent inhibition with low-nanomolar Ki on caspase-1(CARD) polymerization in vitro and inflammasome activation in cells. Whereas caspase-1(CARD) uses six complementary surfaces of three types for filament assembly, INCA is defective in two of the six interfaces and thus terminates the caspase-1 filament.
Molecular basis of caspase-1 polymerization and its inhibition by a new capping mechanism.,Lu A, Li Y, Schmidt FI, Yin Q, Chen S, Fu TM, Tong AB, Ploegh HL, Mao Y, Wu H Nat Struct Mol Biol. 2016 May;23(5):416-25. doi: 10.1038/nsmb.3199. Epub 2016 Apr, 4. PMID:27043298[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Alnemri ES, Fernandes-Alnemri T, Litwack G. Cloning and expression of four novel isoforms of human interleukin-1 beta converting enzyme with different apoptotic activities. J Biol Chem. 1995 Mar 3;270(9):4312-7. PMID:7876192
- ↑ Feng Q, Li P, Leung PC, Auersperg N. Caspase-1zeta, a new splice variant of the caspase-1 gene. Genomics. 2004 Sep;84(3):587-91. PMID:15498465 doi:http://dx.doi.org/S0888-7543(04)00161-2
- ↑ Lu A, Li Y, Schmidt FI, Yin Q, Chen S, Fu TM, Tong AB, Ploegh HL, Mao Y, Wu H. Molecular basis of caspase-1 polymerization and its inhibition by a new capping mechanism. Nat Struct Mol Biol. 2016 May;23(5):416-25. doi: 10.1038/nsmb.3199. Epub 2016 Apr, 4. PMID:27043298 doi:http://dx.doi.org/10.1038/nsmb.3199
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