1lnl
From Proteopedia
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'''Structure of deoxygenated hemocyanin from Rapana thomasiana''' | '''Structure of deoxygenated hemocyanin from Rapana thomasiana''' | ||
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[[Category: Stoeva, S.]] | [[Category: Stoeva, S.]] | ||
[[Category: Voelter, W.]] | [[Category: Voelter, W.]] | ||
| - | [[Category: | + | [[Category: Cooperativity]] |
| - | [[Category: | + | [[Category: Copper]] |
| - | [[Category: | + | [[Category: Deoxygenated form]] |
| - | [[Category: | + | [[Category: Hemocyanin]] |
| - | [[Category: | + | [[Category: Metal binding site]] |
| - | [[Category: | + | [[Category: Oxygen]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:05:30 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 21:05, 2 May 2008
Structure of deoxygenated hemocyanin from Rapana thomasiana
Overview
Structure-function relationships in a molluscan hemocyanin have been investigated by determining the crystal structure of the Rapana thomasiana (gastropod) hemocyanin functional unit RtH2e in deoxygenated form at 3.38 A resolution. This is the first X-ray structure of an unit from the wall of the molluscan hemocyanin cylinder. The crystal structure of RtH2e demonstrates molecular self-assembly of six identical molecules forming a regular hexameric cylinder. This suggests how the functional units are ordered in the wall of the native molluscan hemocyanins. The molecular arrangement is stabilized by specific protomer-to-protomer interactions, which are probably typical for the functional units building the wall of the cylinders. A molecular mechanism for cooperative dioxygen binding in molluscan hemocyanins is proposed on the basis of the molecular interactions between the protomers. In particular, the deoxygenated RtH2e structure reveals a tunnel leading from two opposite sides of the molecule to the active site. The tunnel represents a possible entrance pathway for dioxygen molecules. No such tunnels have been observed in the crystal structure of the oxy-Odg, a functional unit from the Octopus dofleini (cephalopod) hemocyanin in oxygenated form.
About this Structure
1LNL is a Single protein structure of sequence from Rapana thomasiana. Full crystallographic information is available from OCA.
Reference
The structure of a functional unit from the wall of a gastropod hemocyanin offers a possible mechanism for cooperativity., Perbandt M, Guthohrlein EW, Rypniewski W, Idakieva K, Stoeva S, Voelter W, Genov N, Betzel C, Biochemistry. 2003 Jun 3;42(21):6341-6. PMID:12767214 Page seeded by OCA on Sat May 3 00:05:30 2008
