1lni

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[[Image:1lni.gif|left|200px]]
[[Image:1lni.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1lni |SIZE=350|CAPTION= <scene name='initialview01'>1lni</scene>, resolution 1.0&Aring;
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The line below this paragraph, containing "STRUCTURE_1lni", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= U39467 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1894 Streptomyces aureofaciens])
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-->
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|DOMAIN=
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{{STRUCTURE_1lni| PDB=1lni | SCENE= }}
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|RELATEDENTRY=[[1rgg|1RGG]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lni FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lni OCA], [http://www.ebi.ac.uk/pdbsum/1lni PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1lni RCSB]</span>
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}}
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'''CRYSTAL STRUCTURE ANALYSIS OF A RIBONUCLEASE FROM STREPTOMYCES AUREOFACIENS AT ATOMIC RESOLUTION (1.0 A)'''
'''CRYSTAL STRUCTURE ANALYSIS OF A RIBONUCLEASE FROM STREPTOMYCES AUREOFACIENS AT ATOMIC RESOLUTION (1.0 A)'''
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==Reference==
==Reference==
Atomic resolution data reveal flexibility in the structure of RNase Sa., Sevcik J, Lamzin VS, Dauter Z, Wilson KS, Acta Crystallogr D Biol Crystallogr. 2002 Aug;58(Pt 8):1307-13. Epub 2002, Jul 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12136142 12136142]
Atomic resolution data reveal flexibility in the structure of RNase Sa., Sevcik J, Lamzin VS, Dauter Z, Wilson KS, Acta Crystallogr D Biol Crystallogr. 2002 Aug;58(Pt 8):1307-13. Epub 2002, Jul 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12136142 12136142]
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[[Category: Ribonuclease T(1)]]
 
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Streptomyces aureofaciens]]
[[Category: Streptomyces aureofaciens]]
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[[Category: Sevcik, J.]]
[[Category: Sevcik, J.]]
[[Category: Wilson, K S.]]
[[Category: Wilson, K S.]]
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[[Category: hydrolase]]
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[[Category: Hydrolase]]
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[[Category: ribonuclease sa]]
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[[Category: Ribonuclease sa]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:05:32 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:04:57 2008''
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Revision as of 21:05, 2 May 2008

Template:STRUCTURE 1lni

CRYSTAL STRUCTURE ANALYSIS OF A RIBONUCLEASE FROM STREPTOMYCES AUREOFACIENS AT ATOMIC RESOLUTION (1.0 A)


Overview

Ribonuclease from Streptomyces aureofaciens, the bacterial source for the industrial production of chlorotetracycline, is a guanylate endoribonuclease (RNase Sa; EC 3.1.27.3) which hydrolyses the phosphodiester bonds of single-stranded RNA at the 3'-side of guanosine nucleotides with high specificity. The structure of the enzyme was previously refined at atomic resolution (1.2 A) using room-temperature data. Here, the RNase Sa structure refined against 1.0 A data collected at cryogenic temperature is reported. There are two surface loops in molecule A and one in molecule B for which two main-chain conformations are modelled: these loops contain active-site residues. The separation for most of the corresponding main-chain atoms in the two conformations is about 0.8 A, with a maximum of 2.5 A. The two regions of dual conformation represent the most important differences in comparison with the structure determined at room temperature, where the corresponding loops have one conformation only but the largest degree of anisotropy. The flexibility of the loops observed in the structure of RNase Sa is directly linked to the need for the active site to interact productively with substrates and/or inhibitors.

About this Structure

1LNI is a Single protein structure of sequence from Streptomyces aureofaciens. Full crystallographic information is available from OCA.

Reference

Atomic resolution data reveal flexibility in the structure of RNase Sa., Sevcik J, Lamzin VS, Dauter Z, Wilson KS, Acta Crystallogr D Biol Crystallogr. 2002 Aug;58(Pt 8):1307-13. Epub 2002, Jul 20. PMID:12136142 Page seeded by OCA on Sat May 3 00:05:32 2008

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