8i79
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Cryo-EM structure of KCTD7 in complex with Cullin3== | |
| + | <StructureSection load='8i79' size='340' side='right'caption='[[8i79]], [[Resolution|resolution]] 2.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8i79]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8I79 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8I79 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.8Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8i79 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8i79 OCA], [https://pdbe.org/8i79 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8i79 RCSB], [https://www.ebi.ac.uk/pdbsum/8i79 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8i79 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/KCTD7_MOUSE KCTD7_MOUSE] May be involved in the control of excitability of cortical neurons.<ref>PMID:21710140</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | G protein-coupled receptor (GPCR) signaling is precisely controlled to avoid overstimulation that results in detrimental consequences. Gbetagamma signaling is negatively regulated by a Cullin3 (Cul3)-dependent E3 ligase, KCTD5, which triggers ubiquitination and degradation of free Gbetagamma. Here, we report the cryo-electron microscopy structures of the KCTD5-Gbetagamma fusion complex and the KCTD7-Cul3 complex. KCTD5 in pentameric form engages symmetrically with five copies of Gbetagamma through its C-terminal domain. The unique pentameric assembly of the KCTD5/Cul3 E3 ligase places the ubiquitin-conjugating enzyme (E2) and the modification sites of Gbetagamma in close proximity and allows simultaneous transfer of ubiquitin from E2 to five Gbetagamma subunits. Moreover, we show that ubiquitination of Gbetagamma by KCTD5 is important for fine-tuning cyclic adenosine 3 ,5 -monophosphate signaling of GPCRs. Our studies provide unprecedented insights into mechanisms of substrate recognition by unusual pentameric E3 ligases and highlight the KCTD family as emerging regulators of GPCR signaling. | ||
| - | + | Structural basis for the ubiquitination of G protein betagamma subunits by KCTD5/Cullin3 E3 ligase.,Jiang W, Wang W, Kong Y, Zheng S Sci Adv. 2023 Jul 14;9(28):eadg8369. doi: 10.1126/sciadv.adg8369. Epub 2023 Jul , 14. PMID:37450587<ref>PMID:37450587</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Jiang | + | <div class="pdbe-citations 8i79" style="background-color:#fffaf0;"></div> |
| - | [[Category: Wang | + | == References == |
| - | [[Category: Zheng | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Mus musculus]] | ||
| + | [[Category: Jiang W]] | ||
| + | [[Category: Wang W]] | ||
| + | [[Category: Zheng S]] | ||
Current revision
Cryo-EM structure of KCTD7 in complex with Cullin3
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