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| | ==R3/I5 relaxin chimera== | | ==R3/I5 relaxin chimera== |
| - | <StructureSection load='2k1v' size='340' side='right'caption='[[2k1v]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2k1v' size='340' side='right'caption='[[2k1v]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2k1v]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K1V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2K1V FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2k1v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K1V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2K1V FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2fhw|2fhw]]</div></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2k1v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k1v OCA], [https://pdbe.org/2k1v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2k1v RCSB], [https://www.ebi.ac.uk/pdbsum/2k1v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2k1v ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2k1v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k1v OCA], [https://pdbe.org/2k1v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2k1v RCSB], [https://www.ebi.ac.uk/pdbsum/2k1v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2k1v ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/REL3_HUMAN REL3_HUMAN]] May play a role in neuropeptide signaling processes. Ligand for LGR7, relaxin-3 receptor-1 (GPCR135) and relaxin-3 receptor-2 (GPCR142). [[https://www.uniprot.org/uniprot/INSL5_HUMAN INSL5_HUMAN]] May have a role in gut contractility or in thymic development and regulation. Activates RXFP4 with high potency and appears to be the endogenous ligand for this receptor.
| + | [https://www.uniprot.org/uniprot/REL3_HUMAN REL3_HUMAN] May play a role in neuropeptide signaling processes. Ligand for LGR7, relaxin-3 receptor-1 (GPCR135) and relaxin-3 receptor-2 (GPCR142). |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Haugaard-Jonsson, L M]] | + | [[Category: Haugaard-Jonsson LM]] |
| - | [[Category: Rosengren, K]] | + | [[Category: Rosengren K]] |
| - | [[Category: Chimera]]
| + | |
| - | [[Category: Cleavage on pair of basic residue]]
| + | |
| - | [[Category: Hormone]]
| + | |
| - | [[Category: Insl5]]
| + | |
| - | [[Category: Peptide hormone]]
| + | |
| - | [[Category: Relaxin-3]]
| + | |
| - | [[Category: Secreted]]
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| - | [[Category: Signaling protein]]
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| Structural highlights
Function
REL3_HUMAN May play a role in neuropeptide signaling processes. Ligand for LGR7, relaxin-3 receptor-1 (GPCR135) and relaxin-3 receptor-2 (GPCR142).
Publication Abstract from PubMed
The human relaxin family comprises seven peptide hormones with various biological functions mediated through interactions with G-protein-coupled receptors. Interestingly, among the hitherto characterized receptors there is no absolute selectivity toward their primary ligand. The most striking example of this is the relaxin family ancestor, relaxin-3, which is an agonist for three of the four currently known relaxin receptors: GPCR135, GPCR142, and LGR7. Relaxin-3 and its endogenous receptor GPCR135 are both expressed predominantly in the brain and have been linked to regulation of stress and feeding. However, to fully understand the role of relaxin-3 in neurological signaling, the development of selective GPCR135 agonists and antagonists for in vivo studies is crucial. Recent reports have demonstrated that such selective ligands can be achieved by making chimeric peptides comprising the relaxin-3 B-chain combined with the INSL5 A-chain. To obtain structural insights into the consequences of combining A- and B-chains from different relaxins we have determined the NMR solution structure of a human relaxin-3/INSL5 chimeric peptide. The structure reveals that the INSL5 A-chain adopts a conformation similar to the relaxin-3 A-chain, and thus has the ability to structurally support a native-like conformation of the relaxin-3 B-chain. These findings suggest that the decrease in activity at the LGR7 receptor seen for this peptide is a result of the removal of a secondary LGR7 binding site present in the relaxin-3 A-chain, rather than conformational changes in the primary B-chain receptor binding site.
Structure of the R3/I5 chimeric relaxin peptide, a selective GPCR135 and GPCR142 agonist.,Haugaard-Jonsson LM, Hossain MA, Daly NL, Bathgate RA, Wade JD, Craik DJ, Rosengren KJ J Biol Chem. 2008 Aug 29;283(35):23811-8. Epub 2008 Jun 24. PMID:18577524[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Haugaard-Jonsson LM, Hossain MA, Daly NL, Bathgate RA, Wade JD, Craik DJ, Rosengren KJ. Structure of the R3/I5 chimeric relaxin peptide, a selective GPCR135 and GPCR142 agonist. J Biol Chem. 2008 Aug 29;283(35):23811-8. Epub 2008 Jun 24. PMID:18577524 doi:http://dx.doi.org/M800489200
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