2l3n

From Proteopedia

(Difference between revisions)

Revision as of 13:08, 26 July 2023

Solution structure of Rap1-Taz1 fusion protein

Structural highlights

2l3n is a 1 chain structure with sequence from Schizosaccharomyces pombe 972h-. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TAZ1_SCHPO Regulates telomere length and function. Required for the repression of telomere-adjacent gene expression and for normal meiosis or sporulation. It may be a negative regulator of the telomere-replicating enzyme, telomerase, or may protect against activation of telomerase-independent pathways of telomere elongation. It may be involved in the interactions between chromosomes and spindle proteins, disruption of these interactions would lead to defective meiosis.RAP1_SCHPO Involved in the regulation of telomere length, clustering and has a specific role in telomere position effect (TPE). Unlike yeast, exhibits no effect in transcription regulation.^[1] ^[2] ^[3]

Publication Abstract from PubMed

Repressor activator protein 1 (RAP1) is the most highly conserved telomere protein. It is involved in protecting chromosome ends in fission yeast and promoting gene silencing in Saccharomyces cerevisiae, whereas it represses homology-directed recombination at telomeres in mammals. To understand how RAP1 has such diverse functions at telomeres, we solved the crystal or solution structures of the RAP1 C-terminal (RCT) domains of RAP1 from multiple organisms in complex with their respective protein-binding partners. Our analysis establishes RAP1(RCT) as an evolutionarily conserved protein-protein interaction module. In mammalian and fission yeast cells, this module interacts with TRF2 and Taz1, respectively, targeting RAP1 to chromosome ends for telomere protection. In contrast, S. cerevisiae RAP1 uses its RCT domain to recruit Sir3 to telomeres to mediate gene silencing. Together, our results show that, depending on the organism, the evolutionarily conserved RAP1 RCT motif has diverse functional roles at telomeres.

A conserved motif within RAP1 has diversified roles in telomere protection and regulation in different organisms.,Chen Y, Rai R, Zhou ZR, Kanoh J, Ribeyre C, Yang Y, Zheng H, Damay P, Wang F, Tsujii H, Hiraoka Y, Shore D, Hu HY, Chang S, Lei M Nat Struct Mol Biol. 2011 Jan 9. PMID:21217703^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Chikashige Y, Hiraoka Y. Telomere binding of the Rap1 protein is required for meiosis in fission yeast. Curr Biol. 2001 Oct 16;11(20):1618-23. PMID:11676924
↑ Kanoh J, Ishikawa F. spRap1 and spRif1, recruited to telomeres by Taz1, are essential for telomere function in fission yeast. Curr Biol. 2001 Oct 16;11(20):1624-30. PMID:11676925
↑ Chikashige Y, Yamane M, Okamasa K, Tsutsumi C, Kojidani T, Sato M, Haraguchi T, Hiraoka Y. Membrane proteins Bqt3 and -4 anchor telomeres to the nuclear envelope to ensure chromosomal bouquet formation. J Cell Biol. 2009 Nov 2;187(3):413-27. doi: 10.1083/jcb.200902122. PMID:19948484 doi:http://dx.doi.org/10.1083/jcb.200902122
↑ Chen Y, Rai R, Zhou ZR, Kanoh J, Ribeyre C, Yang Y, Zheng H, Damay P, Wang F, Tsujii H, Hiraoka Y, Shore D, Hu HY, Chang S, Lei M. A conserved motif within RAP1 has diversified roles in telomere protection and regulation in different organisms. Nat Struct Mol Biol. 2011 Jan 9. PMID:21217703 doi:10.1038/nsmb.1974

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2l3n"

@@ Line 1: / Line 1: @@
 ==Solution structure of Rap1-Taz1 fusion protein==
-<StructureSection load='2l3n' size='340' side='right'caption='[[2l3n]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='2l3n' size='340' side='right'caption='[[2l3n]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2l3n]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Fission_yeast Fission yeast]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L3N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L3N FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2l3n]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L3N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L3N FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">taz1, myb, myb1, SPAC16A10.07c ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l3n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l3n OCA], [https://pdbe.org/2l3n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l3n RCSB], [https://www.ebi.ac.uk/pdbsum/2l3n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l3n ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/RAP1_SCHPO RAP1_SCHPO]] Involved in the regulation of telomere length, clustering and has a specific role in telomere position effect (TPE). Unlike yeast, exhibits no effect in transcription regulation.<ref>PMID:11676924</ref> <ref>PMID:11676925</ref> <ref>PMID:19948484</ref>
+[https://www.uniprot.org/uniprot/TAZ1_SCHPO TAZ1_SCHPO] Regulates telomere length and function. Required for the repression of telomere-adjacent gene expression and for normal meiosis or sporulation. It may be a negative regulator of the telomere-replicating enzyme, telomerase, or may protect against activation of telomerase-independent pathways of telomere elongation. It may be involved in the interactions between chromosomes and spindle proteins, disruption of these interactions would lead to defective meiosis.[https://www.uniprot.org/uniprot/RAP1_SCHPO RAP1_SCHPO] Involved in the regulation of telomere length, clustering and has a specific role in telomere position effect (TPE). Unlike yeast, exhibits no effect in transcription regulation.<ref>PMID:11676924</ref> <ref>PMID:11676925</ref> <ref>PMID:19948484</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Fission yeast]]
 [[Category: Large Structures]]
-[[Category: Chen, Y]]
+[[Category: Schizosaccharomyces pombe 972h-]]
-[[Category: Hu, H]]
+[[Category: Chen Y]]
-[[Category: Lei, M]]
+[[Category: Hu H]]
-[[Category: Wang, F]]
+[[Category: Lei M]]
-[[Category: Zhou, Z R]]
+[[Category: Wang F]]
-[[Category: Dna binding protein]]
+[[Category: Zhou ZR]]
-[[Category: Rap1]]
-[[Category: Taz1]]

2l3n

From Proteopedia

Revision as of 13:08, 26 July 2023

Solution structure of Rap1-Taz1 fusion protein

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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