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| | <StructureSection load='5frs' size='340' side='right'caption='[[5frs]], [[Resolution|resolution]] 4.07Å' scene=''> | | <StructureSection load='5frs' size='340' side='right'caption='[[5frs]], [[Resolution|resolution]] 4.07Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5frs]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FRS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5FRS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5frs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FRS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FRS FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5frp|5frp]], [[5frr|5frr]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4.073Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5frs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5frs OCA], [http://pdbe.org/5frs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5frs RCSB], [http://www.ebi.ac.uk/pdbsum/5frs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5frs ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5frs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5frs OCA], [https://pdbe.org/5frs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5frs RCSB], [https://www.ebi.ac.uk/pdbsum/5frs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5frs ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PDS5_YEAST PDS5_YEAST]] Essential for the establishment and maintenance of sister chromatid cohesion at centromere proximal and distal regions during S phase. Also required for chromosomal condensation.<ref>PMID:11062262</ref> <ref>PMID:11137006</ref> [[http://www.uniprot.org/uniprot/SCC1_YEAST SCC1_YEAST]] Cleavable component of the cohesin complex involved in chromosome cohesion during cell cycle. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by ESP1 and dissociates from chromatin, allowing sister chromatids to segregate.<ref>PMID:9335333</ref> <ref>PMID:9335334</ref> <ref>PMID:9491073</ref> <ref>PMID:10403247</ref> | + | [https://www.uniprot.org/uniprot/PDS5_YEAST PDS5_YEAST] Essential for the establishment and maintenance of sister chromatid cohesion at centromere proximal and distal regions during S phase. Also required for chromosomal condensation.<ref>PMID:11062262</ref> <ref>PMID:11137006</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 18824]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| | [[Category: Saccharomyces cerevisiae]] | | [[Category: Saccharomyces cerevisiae]] |
| - | [[Category: Haering, C H]] | + | [[Category: Haering CH]] |
| - | [[Category: Kschonsak, M]] | + | [[Category: Kschonsak M]] |
| - | [[Category: Li, Y]] | + | [[Category: Li Y]] |
| - | [[Category: Metz, J]] | + | [[Category: Metz J]] |
| - | [[Category: Muir, K W]] | + | [[Category: Muir KW]] |
| - | [[Category: Panne, D]] | + | [[Category: Panne D]] |
| - | [[Category: Cell cycle]]
| + | |
| - | [[Category: Cohesin]]
| + | |
| - | [[Category: Dna replication]]
| + | |
| - | [[Category: Pds5]]
| + | |
| - | [[Category: Scc1]]
| + | |
| - | [[Category: Sister chromatid cohesion]]
| + | |
| - | [[Category: Smc1]]
| + | |
| - | [[Category: Smc3]]
| + | |
| - | [[Category: Wapl]]
| + | |
| Structural highlights
Function
PDS5_YEAST Essential for the establishment and maintenance of sister chromatid cohesion at centromere proximal and distal regions during S phase. Also required for chromosomal condensation.[1] [2]
Publication Abstract from PubMed
Sister chromatid cohesion is a fundamental prerequisite to faithful genome segregation. Cohesion is precisely regulated by accessory factors that modulate the stability with which the cohesin complex embraces chromosomes. One of these factors, Pds5, engages cohesin through Scc1 and is both a facilitator of cohesion, and, conversely also mediates the release of cohesin from chromatin. We present here the crystal structure of a complex between budding yeast Pds5 and Scc1, thus elucidating the molecular basis of Pds5 function. Pds5 forms an elongated HEAT repeat that binds to Scc1 via a conserved surface patch. We demonstrate that the integrity of the Pds5-Scc1 interface is indispensable for the recruitment of Pds5 to cohesin, and that its abrogation results in loss of sister chromatid cohesion and cell viability.
Structure of the Pds5-Scc1 Complex and Implications for Cohesin Function.,Muir KW, Kschonsak M, Li Y, Metz J, Haering CH, Panne D Cell Rep. 2016 Feb 24. pii: S2211-1247(16)30094-8. doi:, 10.1016/j.celrep.2016.01.078. PMID:26923589[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Hartman T, Stead K, Koshland D, Guacci V. Pds5p is an essential chromosomal protein required for both sister chromatid cohesion and condensation in Saccharomyces cerevisiae. J Cell Biol. 2000 Oct 30;151(3):613-26. PMID:11062262
- ↑ Panizza S, Tanaka T, Hochwagen A, Eisenhaber F, Nasmyth K. Pds5 cooperates with cohesin in maintaining sister chromatid cohesion. Curr Biol. 2000 Dec 14-28;10(24):1557-64. PMID:11137006
- ↑ Muir KW, Kschonsak M, Li Y, Metz J, Haering CH, Panne D. Structure of the Pds5-Scc1 Complex and Implications for Cohesin Function. Cell Rep. 2016 Feb 24. pii: S2211-1247(16)30094-8. doi:, 10.1016/j.celrep.2016.01.078. PMID:26923589 doi:http://dx.doi.org/10.1016/j.celrep.2016.01.078
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