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| | <StructureSection load='5fsc' size='340' side='right'caption='[[5fsc]], [[Resolution|resolution]] 1.95Å' scene=''> | | <StructureSection load='5fsc' size='340' side='right'caption='[[5fsc]], [[Resolution|resolution]] 1.95Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5fsc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bicoloured_deceiver Bicoloured deceiver]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FSC OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5FSC FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5fsc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Laccaria_bicolor_S238N-H82 Laccaria bicolor S238N-H82]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FSC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FSC FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G8Z:ALLYL-ALPHA-4-METHYL-MANNOSIDE'>G8Z</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5fsb|5fsb]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G8Z:ALLYL-ALPHA-4-METHYL-MANNOSIDE'>G8Z</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5fsc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fsc OCA], [http://pdbe.org/5fsc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fsc RCSB], [http://www.ebi.ac.uk/pdbsum/5fsc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fsc ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fsc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fsc OCA], [https://pdbe.org/5fsc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fsc RCSB], [https://www.ebi.ac.uk/pdbsum/5fsc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fsc ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/B0CZL6_LACBS B0CZL6_LACBS] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bicoloured deceiver]] | + | [[Category: Laccaria bicolor S238N-H82]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Bleuer, S]] | + | [[Category: Bleuer S]] |
| - | [[Category: Kunzler, M]] | + | [[Category: Kunzler M]] |
| - | [[Category: Sommer, R]] | + | [[Category: Sommer R]] |
| - | [[Category: Titz, A]] | + | [[Category: Titz A]] |
| - | [[Category: Varrot, A]] | + | [[Category: Varrot A]] |
| - | [[Category: Beta-propeller]]
| + | |
| - | [[Category: Lectin]]
| + | |
| - | [[Category: Methylated sugar]]
| + | |
| - | [[Category: Nematoxic]]
| + | |
| - | [[Category: Sugar binding protein]]
| + | |
| Structural highlights
Function
B0CZL6_LACBS
Publication Abstract from PubMed
Innate immunity is the first line of defense against pathogens and predators. To initiate a response, it relies on the detection of invaders, where lectin-carbohydrate interactions play a major role. O-Methylated glycans were previously identified as non-self epitopes and conserved targets for defense effector proteins belonging to the tectonin superfamily. Here, we present two crystal structures of Tectonin 2 from the mushroom Laccaria bicolor in complex with methylated ligands, unraveling the molecular basis for this original specificity. Furthermore, they revealed the formation of a ball-shaped tetramer with 24 binding sites distributed at its surface, resembling a small virus capsid. Based on the crystal structures, a methylation recognition motif was identified and found in the sequence of many tectonins from bacteria to human. Our results support a key role of tectonins in innate defense based on a distinctive and conserved type of lectin-glycan interaction.
Crystal Structures of Fungal Tectonin in Complex with O-Methylated Glycans Suggest Key Role in Innate Immune Defense.,Sommer R, Makshakova ON, Wohlschlager T, Hutin S, Marsh M, Titz A, Kunzler M, Varrot A Structure. 2018 Mar 6;26(3):391-402.e4. doi: 10.1016/j.str.2018.01.003. Epub 2018, Feb 1. PMID:29398527[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Sommer R, Makshakova ON, Wohlschlager T, Hutin S, Marsh M, Titz A, Kunzler M, Varrot A. Crystal Structures of Fungal Tectonin in Complex with O-Methylated Glycans Suggest Key Role in Innate Immune Defense. Structure. 2018 Mar 6;26(3):391-402.e4. doi: 10.1016/j.str.2018.01.003. Epub 2018, Feb 1. PMID:29398527 doi:http://dx.doi.org/10.1016/j.str.2018.01.003
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