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| | <StructureSection load='5fwu' size='340' side='right'caption='[[5fwu]], [[Resolution|resolution]] 2.80Å' scene=''> | | <StructureSection load='5fwu' size='340' side='right'caption='[[5fwu]], [[Resolution|resolution]] 2.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5fwu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FWU OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5FWU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5fwu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FWU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FWU FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5fws|5fws]], [[5fwt|5fwt]], [[5fwv|5fwv]], [[5fww|5fww]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5fwu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fwu OCA], [http://pdbe.org/5fwu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fwu RCSB], [http://www.ebi.ac.uk/pdbsum/5fwu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fwu ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fwu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fwu OCA], [https://pdbe.org/5fwu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fwu RCSB], [https://www.ebi.ac.uk/pdbsum/5fwu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fwu ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/KREM1_HUMAN KREM1_HUMAN]] Receptor for Dickkopf protein. Cooperates with Dickkopf to block Wnt/beta-catenin signaling (By similarity). | + | [https://www.uniprot.org/uniprot/KREM1_HUMAN KREM1_HUMAN] Receptor for Dickkopf protein. Cooperates with Dickkopf to block Wnt/beta-catenin signaling (By similarity). |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Jackson, V A]] | + | [[Category: Jackson VA]] |
| - | [[Category: Jones, E Y]] | + | [[Category: Jones EY]] |
| - | [[Category: Zebisch, M]] | + | [[Category: Zebisch M]] |
| - | [[Category: Cell surface]]
| + | |
| - | [[Category: Membrane protein]]
| + | |
| - | [[Category: Signaling protein]]
| + | |
| - | [[Category: Signalling]]
| + | |
| - | [[Category: Wnt]]
| + | |
| Structural highlights
Function
KREM1_HUMAN Receptor for Dickkopf protein. Cooperates with Dickkopf to block Wnt/beta-catenin signaling (By similarity).
Publication Abstract from PubMed
Kremen 1 and 2 have been identified as co-receptors for Dickkopf (Dkk) proteins, hallmark secreted antagonists of canonical Wnt signaling. We present here three crystal structures of the ectodomain of human Kremen1 (KRM1ECD) at resolutions between 1.9 and 3.2 A. KRM1ECD emerges as a rigid molecule with tight interactions stabilizing a triangular arrangement of its Kringle, WSC, and CUB structural domains. The structures reveal an unpredicted homology of the WSC domain to hepatocyte growth factor. We further report the general architecture of the ternary complex formed by the Wnt co-receptor Lrp5/6, Dkk, and Krm, determined from a low-resolution complex crystal structure between beta-propeller/EGF repeats (PE) 3 and 4 of the Wnt co-receptor LRP6 (LRP6PE3PE4), the cysteine-rich domain 2 (CRD2) of DKK1, and KRM1ECD. DKK1CRD2 is sandwiched between LRP6PE3 and KRM1Kringle-WSC. Modeling studies supported by surface plasmon resonance suggest a direct interaction site between Krm1CUB and Lrp6PE2.
Structure of the Dual-Mode Wnt Regulator Kremen1 and Insight into Ternary Complex Formation with LRP6 and Dickkopf.,Zebisch M, Jackson VA, Zhao Y, Jones EY Structure. 2016 Sep 6;24(9):1599-1605. doi: 10.1016/j.str.2016.06.020. Epub 2016 , Aug 11. PMID:27524201[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Zebisch M, Jackson VA, Zhao Y, Jones EY. Structure of the Dual-Mode Wnt Regulator Kremen1 and Insight into Ternary Complex Formation with LRP6 and Dickkopf. Structure. 2016 Sep 6;24(9):1599-1605. doi: 10.1016/j.str.2016.06.020. Epub 2016 , Aug 11. PMID:27524201 doi:http://dx.doi.org/10.1016/j.str.2016.06.020
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