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5g18

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Direct Observation of Active-site Protonation States in a Class A beta lactamase with a monobactam substrate

Structural highlights

5g18 is a 1 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.1Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

BLT1_ECOLX Has strong cefotaxime-hydrolyzing activity.

Publication Abstract from PubMed

The monobactam antibiotic aztreonam is used to treat cystic fibrosis patients with chronic pulmonary infections colonized by Pseudomonas aeruginosa strains expressing CTX-M extended-spectrum beta-lactamases. The protonation states of active site residues that are responsible for hydrolysis have been determined previously for the apo form of a CTX-M beta-lactamase, but not for a monobactam acyl-enzyme intermediate. Here we used neutron and high-resolution X-ray crystallography to probe the mechanism by which CTX-M extended-spectrum beta-lactamases hydrolyze monobactam antibiotics. In these first reported structures of a class A beta-lactamase in an acyl-enzyme complex with aztreonam we directly observed most of the hydrogen atoms (as deuterium) within the active site. Although Lys 234 is fully protonated in the acyl-intermediate, we find that Lys 73 is neutral. These findings are consistent with Lys 73 being able to serve as a general base during the acylation part of the catalytic mechanism, as previously proposed. SIGNIFICANCE: Since their initial discovery beta-lactams have constantly remained the most widely prescribed class of antibiotics due to their excellent safety, efficacy coupled with their high availability. The emergence and dissemination of bacterial resistance via beta-lactamase enzymes to this class of drug is now a serious problem in the clinic, often leaving few treatment options for infections resulting from multiple drug resistant superbugs. Using high resolution X-ray and neutron diffraction we have experimentally located key hydrogen atoms and been able to probe the exact mechanism by which extended-spectrum beta-lactamases are able to hydrolyze monobactam antibiotics.

Active Site Protonation States in an Acyl-enzyme Intermediate of a Class A beta-lactamase with a Monobactam Substrate.,Vandavasi VG, Langan PS, Weiss KL, Parks JM, Cooper JB, Ginell SL, Coates L Antimicrob Agents Chemother. 2016 Oct 24. pii: AAC.01636-16. PMID:27795378^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Vandavasi VG, Langan PS, Weiss KL, Parks JM, Cooper JB, Ginell SL, Coates L. Active Site Protonation States in an Acyl-enzyme Intermediate of a Class A beta-lactamase with a Monobactam Substrate. Antimicrob Agents Chemother. 2016 Oct 24. pii: AAC.01636-16. PMID:27795378 doi:http://dx.doi.org/10.1128/AAC.01636-16

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5g18"

@@ Line 3: / Line 3: @@
 <StructureSection load='5g18' size='340' side='right'caption='[[5g18]], [[Resolution|resolution]] 1.10&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5g18]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G18 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5G18 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5g18]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G18 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5G18 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AZR:2-({[(1Z)-1-(2-AMINO-1,3-THIAZOL-4-YL)-2-OXO-2-{[(2S,3S)-1-OXO-3-(SULFOAMINO)BUTAN-2-YL]AMINO}ETHYLIDENE]AMINO}OXY)-2-METHYLPROPANOIC+ACID'>AZR</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.1&#8491;</td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AZR:2-({[(1Z)-1-(2-AMINO-1,3-THIAZOL-4-YL)-2-OXO-2-{[(2S,3S)-1-OXO-3-(SULFOAMINO)BUTAN-2-YL]AMINO}ETHYLIDENE]AMINO}OXY)-2-METHYLPROPANOIC+ACID'>AZR</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5g18 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g18 OCA], [http://pdbe.org/5g18 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5g18 RCSB], [http://www.ebi.ac.uk/pdbsum/5g18 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5g18 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5g18 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g18 OCA], [https://pdbe.org/5g18 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5g18 RCSB], [https://www.ebi.ac.uk/pdbsum/5g18 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5g18 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/BLT1_ECOLX BLT1_ECOLX] Has strong cefotaxime-hydrolyzing activity.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 24: / Line 26: @@
 __TOC__
 </StructureSection>
-[[Category: Bacillus coli migula 1895]]
+[[Category: Escherichia coli]]
-[[Category: Beta-lactamase]]
 [[Category: Large Structures]]
-[[Category: Coates, L]]
+[[Category: Coates L]]
-[[Category: Cooper, J B]]
+[[Category: Cooper JB]]
-[[Category: Ginell, S L]]
+[[Category: Ginell SL]]
-[[Category: Parks, J M]]
+[[Category: Parks JM]]
-[[Category: Vandavasi, V G]]
+[[Category: Vandavasi VG]]
-[[Category: Weiss, K L]]
+[[Category: Weiss KL]]
-[[Category: Beta lactamase]]
-[[Category: Hydrolase]]
-[[Category: Neutron]]
-[[Category: Toho]]

5g18

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Current revision

Direct Observation of Active-site Protonation States in a Class A beta lactamase with a monobactam substrate

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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