1avb
From Proteopedia
(Difference between revisions)
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==ARCELIN-1 FROM PHASEOLUS VULGARIS L== | ==ARCELIN-1 FROM PHASEOLUS VULGARIS L== | ||
| - | <StructureSection load='1avb' size='340' side='right'caption='[[1avb]]' scene=''> | + | <StructureSection load='1avb' size='340' side='right'caption='[[1avb]], [[Resolution|resolution]] 1.90Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AVB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AVB FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1avb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Phaseolus_vulgaris Phaseolus vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AVB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AVB FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1avb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1avb OCA], [https://pdbe.org/1avb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1avb RCSB], [https://www.ebi.ac.uk/pdbsum/1avb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1avb ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1avb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1avb OCA], [https://pdbe.org/1avb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1avb RCSB], [https://www.ebi.ac.uk/pdbsum/1avb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1avb ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/ARC1_PHAVU ARC1_PHAVU] Seed storage. This carbohydrate-binding lectin has toxic effects on an important bean bruchid pest, Z.subfasciatus. Antibiosis properties of legume lectins are proposed to be due to the lysis of epithelial cells of the intestine by binding to the carbohydrate moieties of these proteins. | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1avb ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1avb ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Arcelin-1 is a glycoprotein from kidney beans (Phaseolus vulgaris) which displays insecticidal properties and protects the seeds from predation by larvae of various bruchids. This lectin-like protein is devoid of monosaccharide binding properties and belongs to the phytohemagglutinin protein family. The x-ray structure determination at 1.9-A resolution of native arcelin-1 dimers, which correspond to the functional state of the protein in solution, was solved using multiple isomorphous replacement and refined to a crystallographic R factor of 0.208. The three glycosylation sites on each monomer are all covalently modified. One of these oligosaccharide chains provides interactions with protein atoms at the dimer interface, and another one may act by preventing the formation of higher oligomeric species in the arcelin variants. The dimeric structure and the severe alteration of the monosaccharide binding site in arcelin-1 correlate with the hemagglutinating properties of the protein, which are unaffected by simple sugars and sugar derivatives. Sequence analysis and structure comparisons of arcelin-1 with the other insecticidal proteins from kidney beans, arcelin-5, and alpha-amylase inhibitor and with legume lectins, yield insights into the molecular basis of the different biological functions of these proteins. | ||
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| + | Crystal structure of the arcelin-1 dimer from Phaseolus vulgaris at 1.9-A resolution.,Mourey L, Pedelacq JD, Birck C, Fabre C, Rouge P, Samama JP J Biol Chem. 1998 May 22;273(21):12914-22. PMID:9582323<ref>PMID:9582323</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 1avb" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Phaseolus vulgaris]] | ||
[[Category: Fabre C]] | [[Category: Fabre C]] | ||
[[Category: Mourey L]] | [[Category: Mourey L]] | ||
Current revision
ARCELIN-1 FROM PHASEOLUS VULGARIS L
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