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| | <StructureSection load='5gj9' size='340' side='right'caption='[[5gj9]], [[Resolution|resolution]] 2.10Å' scene=''> | | <StructureSection load='5gj9' size='340' side='right'caption='[[5gj9]], [[Resolution|resolution]] 2.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5gj9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GJ9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GJ9 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5gj9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GJ9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5GJ9 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=VGL:PYRAZINE-2-CARBOXYLIC+ACID'>VGL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5gja|5gja]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=VGL:PYRAZINE-2-CARBOXYLIC+ACID'>VGL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ACO2, EI305, At1g62380, F24O1.10 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5gj9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gj9 OCA], [https://pdbe.org/5gj9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5gj9 RCSB], [https://www.ebi.ac.uk/pdbsum/5gj9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5gj9 ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aminocyclopropanecarboxylate_oxidase Aminocyclopropanecarboxylate oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.17.4 1.14.17.4] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5gj9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gj9 OCA], [http://pdbe.org/5gj9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gj9 RCSB], [http://www.ebi.ac.uk/pdbsum/5gj9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gj9 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ACCO2_ARATH ACCO2_ARATH]] Enzyme involved in the ethylene biosynthesis. Required to mediate the 1-aminocyclopropane-1-carboxylic acid (ACC)-mediated reversion of the ABA-induced inhibition of seed germination via endosperm rupture. May promote stem elongation by maximizing the extensibility cells, possibly by activating ethylene biosynthesis, in response to very-long-chain fatty acids (VLCFAs C20:0 to C30:0).<ref>PMID:17993622</ref> <ref>PMID:20023197</ref> | + | [https://www.uniprot.org/uniprot/ACCO2_ARATH ACCO2_ARATH] Enzyme involved in the ethylene biosynthesis. Required to mediate the 1-aminocyclopropane-1-carboxylic acid (ACC)-mediated reversion of the ABA-induced inhibition of seed germination via endosperm rupture. May promote stem elongation by maximizing the extensibility cells, possibly by activating ethylene biosynthesis, in response to very-long-chain fatty acids (VLCFAs C20:0 to C30:0).<ref>PMID:17993622</ref> <ref>PMID:20023197</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Aminocyclopropanecarboxylate oxidase]] | + | [[Category: Arabidopsis thaliana]] |
| - | [[Category: Arath]]
| + | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Du, S]] | + | [[Category: Du S]] |
| - | [[Category: Guo, H W]] | + | [[Category: Guo HW]] |
| - | [[Category: He, W R]] | + | [[Category: He WR]] |
| - | [[Category: Ji, C G]] | + | [[Category: Ji CG]] |
| - | [[Category: Li, H J]] | + | [[Category: Li HJ]] |
| - | [[Category: Li, Y X]] | + | [[Category: Li YX]] |
| - | [[Category: Raikhel, N]] | + | [[Category: Raikhel N]] |
| - | [[Category: Sun, X Z]] | + | [[Category: Sun XZ]] |
| - | [[Category: Wang, Y C]] | + | [[Category: Wang YC]] |
| - | [[Category: Xia, P X]] | + | [[Category: Xia PX]] |
| - | [[Category: Xiao, J Y]] | + | [[Category: Xiao JY]] |
| - | [[Category: Ethylene oxidase inhibitor]]
| + | |
| - | [[Category: Oxidoreductase]]
| + | |
| Structural highlights
Function
ACCO2_ARATH Enzyme involved in the ethylene biosynthesis. Required to mediate the 1-aminocyclopropane-1-carboxylic acid (ACC)-mediated reversion of the ABA-induced inhibition of seed germination via endosperm rupture. May promote stem elongation by maximizing the extensibility cells, possibly by activating ethylene biosynthesis, in response to very-long-chain fatty acids (VLCFAs C20:0 to C30:0).[1] [2]
Publication Abstract from PubMed
Ethylene is an important phytohormone that promotes the ripening of fruits and senescence of flowers thereby reducing their shelf lives. Specific ethylene biosynthesis inhibitors would help to decrease postharvest loss. Here, we identify pyrazinamide (PZA), a clinical drug used to treat tuberculosis, as an inhibitor of ethylene biosynthesis in Arabidopsis thaliana, using a chemical genetics approach. PZA is converted to pyrazinecarboxylic acid (POA) in plant cells, suppressing the activity of 1-aminocyclopropane-1-carboxylic acid oxidase (ACO), the enzyme catalysing the final step of ethylene formation. The crystal structures of Arabidopsis ACO2 in complex with POA or 2-Picolinic Acid (2-PA), a POA-related compound, reveal that POA/2-PA bind at the active site of ACO, preventing the enzyme from interacting with its natural substrates. Our work suggests that PZA and its derivatives may be promising regulators of plant metabolism, in particular ethylene biosynthesis.
Pyrazinamide and derivatives block ethylene biosynthesis by inhibiting ACC oxidase.,Sun X, Li Y, He W, Ji C, Xia P, Wang Y, Du S, Li H, Raikhel N, Xiao J, Guo H Nat Commun. 2017 Jun 12;8:15758. doi: 10.1038/ncomms15758. PMID:28604689[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Qin YM, Hu CY, Pang Y, Kastaniotis AJ, Hiltunen JK, Zhu YX. Saturated very-long-chain fatty acids promote cotton fiber and Arabidopsis cell elongation by activating ethylene biosynthesis. Plant Cell. 2007 Nov;19(11):3692-704. Epub 2007 Nov 9. PMID:17993622 doi:http://dx.doi.org/10.1105/tpc.107.054437
- ↑ Linkies A, Muller K, Morris K, Tureckova V, Wenk M, Cadman CS, Corbineau F, Strnad M, Lynn JR, Finch-Savage WE, Leubner-Metzger G. Ethylene interacts with abscisic acid to regulate endosperm rupture during germination: a comparative approach using Lepidium sativum and Arabidopsis thaliana. Plant Cell. 2009 Dec;21(12):3803-22. doi: 10.1105/tpc.109.070201. Epub 2009 Dec, 18. PMID:20023197 doi:http://dx.doi.org/10.1105/tpc.109.070201
- ↑ Sun X, Li Y, He W, Ji C, Xia P, Wang Y, Du S, Li H, Raikhel N, Xiao J, Guo H. Pyrazinamide and derivatives block ethylene biosynthesis by inhibiting ACC oxidase. Nat Commun. 2017 Jun 12;8:15758. doi: 10.1038/ncomms15758. PMID:28604689 doi:http://dx.doi.org/10.1038/ncomms15758
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