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| | <StructureSection load='5got' size='340' side='right'caption='[[5got]], [[Resolution|resolution]] 1.90Å' scene=''> | | <StructureSection load='5got' size='340' side='right'caption='[[5got]], [[Resolution|resolution]] 1.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5got]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_scarlatinae"_klein_1884 "micrococcus scarlatinae" klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GOT OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5GOT FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5got]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GOT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5GOT FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.902Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ptpA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1314 "Micrococcus scarlatinae" Klein 1884])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5got FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5got OCA], [https://pdbe.org/5got PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5got RCSB], [https://www.ebi.ac.uk/pdbsum/5got PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5got ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5got FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5got OCA], [http://pdbe.org/5got PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5got RCSB], [http://www.ebi.ac.uk/pdbsum/5got PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5got ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q9A1Y0_STRP1 Q9A1Y0_STRP1] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Micrococcus scarlatinae klein 1884]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Protein-tyrosine-phosphatase]] | + | [[Category: Streptococcus pyogenes]] |
| - | [[Category: KIim, S J]] | + | [[Category: KIim SJ]] |
| - | [[Category: Keum, C W]] | + | [[Category: Keum CW]] |
| - | [[Category: Ku, B]] | + | [[Category: Ku B]] |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Lmwptp]]
| + | |
| - | [[Category: Sp-ptp]]
| + | |
| - | [[Category: Streptococcus pyogene]]
| + | |
| Structural highlights
Function
Q9A1Y0_STRP1
Publication Abstract from PubMed
Streptococcus pyogenes, or Group A Streptococcus (GAS), is a pathogenic bacterium that causes a variety of infectious diseases. The GAS genome encodes one protein tyrosine phosphatase, SP-PTP, which plays an essential role in the replication and virulence maintenance of GAS. Herein, we present the crystal structure of SP-PTP at 1.9 A resolution. Although SP-PTP has been reported to have dual phosphatase specificity for both phosphorylated tyrosine and serine/threonine, three-dimensional structural analysis showed that SP-PTP shares high similarity with typical low molecular weight protein tyrosine phosphatases (LMWPTPs), which are specific for phosphotyrosine, but not with dual-specificity phosphatases, in overall folding and active site composition. In the dephosphorylation activity test, SP-PTP consistently acted on phosphotyrosine substrates, but not or only minimally on phosphoserine/phosphothreonine substrates. Collectively, our structural and biochemical analyses verified SP-PTP as a canonical tyrosine-specific LMWPTP.
Crystal structure of SP-PTP, a low molecular weight protein tyrosine phosphatase from Streptococcus pyogenes.,Ku B, Keum CW, Lee HS, Yun HY, Shin HC, Kim BY, Kim SJ Biochem Biophys Res Commun. 2016 Aug 19. pii: S0006-291X(16)31352-3. doi:, 10.1016/j.bbrc.2016.08.097. PMID:27545603[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Ku B, Keum CW, Lee HS, Yun HY, Shin HC, Kim BY, Kim SJ. Crystal structure of SP-PTP, a low molecular weight protein tyrosine phosphatase from Streptococcus pyogenes. Biochem Biophys Res Commun. 2016 Aug 19. pii: S0006-291X(16)31352-3. doi:, 10.1016/j.bbrc.2016.08.097. PMID:27545603 doi:http://dx.doi.org/10.1016/j.bbrc.2016.08.097
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