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| | ==Crystal structure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose== | | ==Crystal structure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose== |
| - | <StructureSection load='5gr4' size='340' side='right' caption='[[5gr4]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='5gr4' size='340' side='right'caption='[[5gr4]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5gr4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cyaa5 Cyaa5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GR4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GR4 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5gr4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Crocosphaera_subtropica_ATCC_51142 Crocosphaera subtropica ATCC 51142]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GR4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5GR4 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5gqu|5gqu]], [[5gqv|5gqv]], [[5gqw|5gqw]], [[5gqx|5gqx]], [[5gqy|5gqy]], [[5gqz|5gqz]], [[5gr0|5gr0]], [[5gr1|5gr1]], [[5gr2|5gr2]], [[5gr3|5gr3]], [[5gr5|5gr5]], [[5gr6|5gr6]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PRD_900001:alpha-maltose'>PRD_900001</scene>, <scene name='pdbligand=PRD_900009:alpha-maltotriose'>PRD_900009</scene>, <scene name='pdbligand=PRD_900010:alpha-maltotetraose'>PRD_900010</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">glgB, glgB1, cce_2248 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=43989 CYAA5])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5gr4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gr4 OCA], [https://pdbe.org/5gr4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5gr4 RCSB], [https://www.ebi.ac.uk/pdbsum/5gr4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5gr4 ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1,4-alpha-glucan_branching_enzyme 1,4-alpha-glucan branching enzyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.18 2.4.1.18] </span></td></tr> | + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5gr4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gr4 OCA], [http://pdbe.org/5gr4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gr4 RCSB], [http://www.ebi.ac.uk/pdbsum/5gr4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gr4 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/B1WPM8_CYAA5 B1WPM8_CYAA5]] Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.[HAMAP-Rule:MF_00685] | + | [https://www.uniprot.org/uniprot/B1WPM8_CROS5 B1WPM8_CROS5] Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.[HAMAP-Rule:MF_00685] |
| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | Several cyanobacterial species, including Cyanothece sp. ATCC 51142, remarkably have four isoforms of alpha-glucan branching enzymes (BEs). Based on their primary structures, they are classified into glycoside hydrolase (GH) family 13 (BE1, BE2 and BE3) or family 57 (GH57 BE). In the present study, GH13-type BEs from Cyanothece sp. ATCC 51142 (BE1, BE2 and BE3) have been overexpressed in Escherichia coli and biochemically characterized. The recombinant BE1 was crystallized by the hanging-drop vapour-diffusion method. Crystals of BE1 were obtained at 293 K in the presence of 0.2 M Mg(2+), 7-10%(w/v) ethanol, 0.1 M HEPES-NaOH pH 7.2-7.9. The crystals belonged to the tetragonal space group P41212, with unit-cell parameters a = b = 133.75, c = 185.90 A, and diffracted to beyond 1.85 A resolution. Matthews coefficient calculations suggested that the crystals of BE1 contained two molecules in the asymmetric unit.
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| - | Crystallization and crystallographic analysis of branching enzymes from Cyanothece sp. ATCC 51142.,Hayashi M, Suzuki R, Colleoni C, Ball SG, Fujita N, Suzuki E Acta Crystallogr F Struct Biol Commun. 2015 Aug;71(Pt 8):1109-13. doi:, 10.1107/S2053230X1501198X. Epub 2015 Jul 29. PMID:26249708<ref>PMID:26249708</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 5gr4" style="background-color:#fffaf0;"></div>
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| - | == References ==
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| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: 1,4-alpha-glucan branching enzyme]] | + | [[Category: Crocosphaera subtropica ATCC 51142]] |
| - | [[Category: Cyaa5]] | + | [[Category: Large Structures]] |
| - | [[Category: Suzuki, E]] | + | [[Category: Suzuki E]] |
| - | [[Category: Suzuki, R]] | + | [[Category: Suzuki R]] |
| - | [[Category: Branching enzyme]]
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| - | [[Category: Cyanobacteria]]
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| - | [[Category: Glycoside hydrolase family 13]]
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| - | [[Category: Starch]]
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| - | [[Category: Transferase]]
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