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| | <SX load='5gsy' size='340' side='right' viewer='molstar' caption='[[5gsy]], [[Resolution|resolution]] 7.00Å' scene=''> | | <SX load='5gsy' size='340' side='right' viewer='molstar' caption='[[5gsy]], [[Resolution|resolution]] 7.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5gsy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GSY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5GSY FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5gsy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GSY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5GSY FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Kif19, Kif19a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 7Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5gsy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gsy OCA], [http://pdbe.org/5gsy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gsy RCSB], [http://www.ebi.ac.uk/pdbsum/5gsy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gsy ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5gsy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gsy OCA], [https://pdbe.org/5gsy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5gsy RCSB], [https://www.ebi.ac.uk/pdbsum/5gsy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5gsy ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/KIF19_MOUSE KIF19_MOUSE]] Plus end-directed microtubule-dependent motor protein that regulates the length of motile cilia by mediating depolymerization of microtubules at ciliary tips.<ref>PMID:23168168</ref> | + | [https://www.uniprot.org/uniprot/KIF19_MOUSE KIF19_MOUSE] Plus end-directed microtubule-dependent motor protein that regulates the length of motile cilia by mediating depolymerization of microtubules at ciliary tips.<ref>PMID:23168168</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </SX> | | </SX> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Mus musculus]] |
| - | [[Category: Hirokawa, N]] | + | [[Category: Hirokawa N]] |
| - | [[Category: Kikkawa, M]] | + | [[Category: Kikkawa M]] |
| - | [[Category: Morikawa, M]] | + | [[Category: Morikawa M]] |
| - | [[Category: Nitta, R]] | + | [[Category: Nitta R]] |
| - | [[Category: Shigematsu, H]] | + | [[Category: Shigematsu H]] |
| - | [[Category: Yajima, H]] | + | [[Category: Yajima H]] |
| - | [[Category: Gdp-taxol-microtubule]]
| + | |
| - | [[Category: Kif19a]]
| + | |
| - | [[Category: Kinesin-8]]
| + | |
| - | [[Category: Microtubule depolymerization]]
| + | |
| - | [[Category: Motor protein]]
| + | |
| - | [[Category: Plus-end directed motor]]
| + | |
| Structural highlights
Function
KIF19_MOUSE Plus end-directed microtubule-dependent motor protein that regulates the length of motile cilia by mediating depolymerization of microtubules at ciliary tips.[1]
Publication Abstract from PubMed
The kinesin-8 motor, KIF19A, accumulates at cilia tips and controls cilium length. Defective KIF19A leads to hydrocephalus and female infertility because of abnormally elongated cilia. Uniquely among kinesins, KIF19A possesses the dual functions of motility along ciliary microtubules and depolymerization of microtubules. To elucidate the molecular mechanisms of these functions we solved the crystal structure of its motor domain and determined its cryo-electron microscopy structure complexed with a microtubule. The features of KIF19A that enable its dual function are clustered on its microtubule-binding side. Unexpectedly, a destabilized switch II coordinates with a destabilized L8 to enable KIF19A to adjust to both straight and curved microtubule protofilaments. The basic clusters of L2 and L12 tether the microtubule. The long L2 with a characteristic acidic-hydrophobic-basic sequence effectively stabilizes the curved conformation of microtubule ends. Hence, KIF19A utilizes multiple strategies to accomplish the dual functions of motility and microtubule depolymerization by ATP hydrolysis.
Motility and microtubule depolymerization mechanisms of the Kinesin-8 motor, KIF19A.,Wang D, Nitta R, Morikawa M, Yajima H, Inoue S, Shigematsu H, Kikkawa M, Hirokawa N Elife. 2016 Sep 30;5. pii: e18101. doi: 10.7554/eLife.18101. PMID:27690357[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Niwa S, Nakajima K, Miki H, Minato Y, Wang D, Hirokawa N. KIF19A is a microtubule-depolymerizing kinesin for ciliary length control. Dev Cell. 2012 Dec 11;23(6):1167-75. doi: 10.1016/j.devcel.2012.10.016. Epub 2012, Nov 15. PMID:23168168 doi:http://dx.doi.org/10.1016/j.devcel.2012.10.016
- ↑ Wang D, Nitta R, Morikawa M, Yajima H, Inoue S, Shigematsu H, Kikkawa M, Hirokawa N. Motility and microtubule depolymerization mechanisms of the Kinesin-8 motor, KIF19A. Elife. 2016 Sep 30;5. pii: e18101. doi: 10.7554/eLife.18101. PMID:27690357 doi:http://dx.doi.org/10.7554/eLife.18101
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