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| | ==Crystal structure of human RCC2== | | ==Crystal structure of human RCC2== |
| - | <StructureSection load='5gwn' size='340' side='right' caption='[[5gwn]], [[Resolution|resolution]] 1.31Å' scene=''> | + | <StructureSection load='5gwn' size='340' side='right'caption='[[5gwn]], [[Resolution|resolution]] 1.31Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5gwn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GWN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GWN FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5gwn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GWN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5GWN FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.309Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RCC2, KIAA1470, TD60 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5gwn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gwn OCA], [http://pdbe.org/5gwn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gwn RCSB], [http://www.ebi.ac.uk/pdbsum/5gwn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gwn ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5gwn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gwn OCA], [https://pdbe.org/5gwn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5gwn RCSB], [https://www.ebi.ac.uk/pdbsum/5gwn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5gwn ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/RCC2_HUMAN RCC2_HUMAN]] Required for completion of mitosis and cytokinesis. May function as a guanine nucleotide exchange factor for the small GTPase RAC1.<ref>PMID:12919680</ref> | + | [https://www.uniprot.org/uniprot/RCC2_HUMAN RCC2_HUMAN] Required for completion of mitosis and cytokinesis. May function as a guanine nucleotide exchange factor for the small GTPase RAC1.<ref>PMID:12919680</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Liang, L]] | + | [[Category: Large Structures]] |
| - | [[Category: Yin, Y X]] | + | [[Category: Liang L]] |
| - | [[Category: Yun, C H]] | + | [[Category: Yin YX]] |
| - | [[Category: Protein binding]] | + | [[Category: Yun CH]] |
| - | [[Category: Seven-bladed propeller]]
| + | |
| Structural highlights
Function
RCC2_HUMAN Required for completion of mitosis and cytokinesis. May function as a guanine nucleotide exchange factor for the small GTPase RAC1.[1]
Publication Abstract from PubMed
RCC2 (also known as TD60) is a highly conserved protein involved in prognosis in colorectal cancer. However, its relationship with tumor development is less understood. Here we demonstrate a signaling pathway defining regulation of RCC2 and its functions in tumor progression. We report that p53 is a transcriptional regulator of RCC2 that acts through its binding to a palindromic motif in the RCC2 promoter. RCC2 physically interacts and deactivates a small GTPase Rac1 that is known to be involved in metastasis. We solved a high-resolution crystal structure of RCC2 and revealed one RCC1-like domain with a unique beta-hairpin that is requisite for RCC2 interaction with Rac1. p53 or RCC2 deficiency leads to activation of Rac1 and deterioration of extracellular matrix sensing (haptotaxis) of surface-bound gradients. Ectopic expression of RCC2 restores directional migration in p53-null cells. Our results demonstrate that p53 and RCC2 signaling is important for regulation of cell migration and suppression of metastasis. We propose that the p53/RCC2/Rac1 axis is a potential target for cancer therapy.Oncogene advance online publication, 4 September 2017; doi:10.1038/onc.2017.306.
RCC2 is a novel p53 target in suppressing metastasis.,Song C, Liang L, Jin Y, Li Y, Liu Y, Guo L, Wu C, Yun CH, Yin Y Oncogene. 2017 Sep 4. doi: 10.1038/onc.2017.306. PMID:28869598[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Mollinari C, Reynaud C, Martineau-Thuillier S, Monier S, Kieffer S, Garin J, Andreassen PR, Boulet A, Goud B, Kleman JP, Margolis RL. The mammalian passenger protein TD-60 is an RCC1 family member with an essential role in prometaphase to metaphase progression. Dev Cell. 2003 Aug;5(2):295-307. PMID:12919680
- ↑ Song C, Liang L, Jin Y, Li Y, Liu Y, Guo L, Wu C, Yun CH, Yin Y. RCC2 is a novel p53 target in suppressing metastasis. Oncogene. 2017 Sep 4. doi: 10.1038/onc.2017.306. PMID:28869598 doi:http://dx.doi.org/10.1038/onc.2017.306
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