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| | <StructureSection load='5gxb' size='340' side='right'caption='[[5gxb]], [[Resolution|resolution]] 3.30Å' scene=''> | | <StructureSection load='5gxb' size='340' side='right'caption='[[5gxb]], [[Resolution|resolution]] 3.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5gxb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Alpaca Alpaca] and [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GXB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GXB FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5gxb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12] and [https://en.wikipedia.org/wiki/Vicugna_pacos Vicugna pacos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GXB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5GXB FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lacY, b0343, JW0334 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5gxb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gxb OCA], [http://pdbe.org/5gxb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gxb RCSB], [http://www.ebi.ac.uk/pdbsum/5gxb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gxb ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5gxb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gxb OCA], [https://pdbe.org/5gxb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5gxb RCSB], [https://www.ebi.ac.uk/pdbsum/5gxb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5gxb ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/LACY_ECOLI LACY_ECOLI]] Responsible for transport of beta-galactosides into the cell, with the concomitant import of a proton (symport system). | + | [https://www.uniprot.org/uniprot/LACY_ECOLI LACY_ECOLI] Responsible for transport of beta-galactosides into the cell, with the concomitant import of a proton (symport system). |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Alpaca]] | + | [[Category: Escherichia coli K-12]] |
| - | [[Category: Ecoli]]
| + | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Jiang, X]] | + | [[Category: Vicugna pacos]] |
| - | [[Category: Kaback, H R]] | + | [[Category: Jiang X]] |
| - | [[Category: Wu, J P]] | + | [[Category: Kaback HR]] |
| - | [[Category: Yan, N]] | + | [[Category: Wu JP]] |
| - | [[Category: Transport protein]] | + | [[Category: Yan N]] |
| - | [[Category: Transporter]]
| + | |
| Structural highlights
Function
LACY_ECOLI Responsible for transport of beta-galactosides into the cell, with the concomitant import of a proton (symport system).
Publication Abstract from PubMed
The lactose permease of Escherichia coli (LacY), a dynamic polytopic membrane protein, catalyzes galactoside-H+ symport and operates by an alternating access mechanism that exhibits multiple conformations, the distribution of which is altered by sugar binding. We have developed single-domain camelid nanobodies (Nbs) against a mutant in an outward (periplasmic)-open conformation to stabilize this state of the protein. Here we describe an X-ray crystal structure of a complex between a double-Trp mutant (Gly46-->Trp/Gly262-->Trp) and an Nb in which free access to the sugar-binding site from the periplasmic cavity is observed. The structure confirms biochemical data indicating that the Nb binds stoichiometrically with nanomolar affinity to the periplasmic face of LacY primarily to the C-terminal six-helix bundle. The structure is novel because the pathway to the sugar-binding site is constricted and the central cavity containing the galactoside-binding site is empty. Although Phe27 narrows the periplasmic cavity, sugar is freely accessible to the binding site. Remarkably, the side chains directly involved in binding galactosides remain in the same position in the absence or presence of bound sugar.
Crystal structure of a LacY-nanobody complex in a periplasmic-open conformation.,Jiang X, Smirnova I, Kasho V, Wu J, Hirata K, Ke M, Pardon E, Steyaert J, Yan N, Kaback HR Proc Natl Acad Sci U S A. 2016 Nov 1;113(44):12420-12425. Epub 2016 Oct 19. PMID:27791182[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Jiang X, Smirnova I, Kasho V, Wu J, Hirata K, Ke M, Pardon E, Steyaert J, Yan N, Kaback HR. Crystal structure of a LacY-nanobody complex in a periplasmic-open conformation. Proc Natl Acad Sci U S A. 2016 Nov 1;113(44):12420-12425. Epub 2016 Oct 19. PMID:27791182 doi:http://dx.doi.org/10.1073/pnas.1615414113
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