1lt9
From Proteopedia
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[[Image:1lt9.jpg|left|200px]] | [[Image:1lt9.jpg|left|200px]] | ||
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'''Crystal Structure of Recombinant Human Fibrinogen Fragment D''' | '''Crystal Structure of Recombinant Human Fibrinogen Fragment D''' | ||
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[[Category: Kostelansky, M S.]] | [[Category: Kostelansky, M S.]] | ||
[[Category: Lord, S T.]] | [[Category: Lord, S T.]] | ||
| - | [[Category: | + | [[Category: Blood coagulation]] |
| - | [[Category: | + | [[Category: Fibrinogen]] |
| - | [[Category: | + | [[Category: Fibrinogen fragment d]] |
| - | [[Category: | + | [[Category: Recombinant fibrinogen]] |
| - | [[Category: | + | [[Category: Recombinant fibrinogen fragment d]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:15:53 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 21:15, 2 May 2008
Crystal Structure of Recombinant Human Fibrinogen Fragment D
Overview
We report two crystal structures, each at a resolution of 2.8 A, of recombinant human fibrinogen fragment D (rfD) in the absence and presence of peptide ligands. The bound ligands, Gly-Pro-Arg-Pro-amide and Gly-His-Arg-Pro-amide, mimic the interactions of the thrombin exposed polymerization sites, "A" and "B", respectively. This report is the first to describe the structure of fragment D in the presence of both peptide ligands. The structures reveal that recombinant fibrinogen is nearly identical to the plasma protein but with minor changes, like the addition of a proximal fucose to the carbohydrate linked to residue betaGln364, and slightly different relative positions of the beta- and gamma-modules. Of major interest in our structures is that a previously identified calcium site in plasma fibrinogen is absent when Gly-His-Arg-Pro-amide is bound. The peptide-dependent loss of this calcium site may have significant biological implications that are further discussed. These structures provide a foundation for the detailed structural analysis of variant recombinant fibrinogens that were used to identify critical functional residues within fragment D.
About this Structure
1LT9 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
2.8 A crystal structures of recombinant fibrinogen fragment D with and without two peptide ligands: GHRP binding to the "b" site disrupts its nearby calcium-binding site., Kostelansky MS, Betts L, Gorkun OV, Lord ST, Biochemistry. 2002 Oct 8;41(40):12124-32. PMID:12356313 Page seeded by OCA on Sat May 3 00:15:53 2008
