1lua
From Proteopedia
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'''Structure of methylene-tetrahydromethanopterin dehydrogenase from Methylobacterium extorquens AM1 complexed with NADP''' | '''Structure of methylene-tetrahydromethanopterin dehydrogenase from Methylobacterium extorquens AM1 complexed with NADP''' | ||
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==Reference== | ==Reference== | ||
Structure of methylene-tetrahydromethanopterin dehydrogenase from methylobacterium extorquens AM1., Ermler U, Hagemeier CH, Roth A, Demmer U, Grabarse W, Warkentin E, Vorholt JA, Structure. 2002 Aug;10(8):1127-37. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12176390 12176390] | Structure of methylene-tetrahydromethanopterin dehydrogenase from methylobacterium extorquens AM1., Ermler U, Hagemeier CH, Roth A, Demmer U, Grabarse W, Warkentin E, Vorholt JA, Structure. 2002 Aug;10(8):1127-37. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12176390 12176390] | ||
| - | [[Category: Methylenetetrahydrofolate dehydrogenase (NADP(+))]] | ||
[[Category: Methylobacterium extorquens]] | [[Category: Methylobacterium extorquens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Vorholt, J A.]] | [[Category: Vorholt, J A.]] | ||
[[Category: Warkentin, E.]] | [[Category: Warkentin, E.]] | ||
| - | [[Category: | + | [[Category: Alpha/beta twisted open sheet structure]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:17:44 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 21:17, 2 May 2008
Structure of methylene-tetrahydromethanopterin dehydrogenase from Methylobacterium extorquens AM1 complexed with NADP
Overview
NADP-dependent methylene-H(4)MPT dehydrogenase, MtdA, from Methylobacterium extorquens AM1 catalyzes the dehydrogenation of methylene-tetrahydromethanopterin and methylene-tetrahydrofolate with NADP(+) as cosubstrate. The X-ray structure of MtdA with and without NADP bound was established at 1.9 A resolution. The enzyme is present as a homotrimer. The alpha,beta fold of the monomer is related to that of methylene-H(4)F dehydrogenases, suggesting a common evolutionary origin. The position of the active site is located within a large crevice built up by the two domains of one subunit and one domain of a second subunit. Methylene-H(4)MPT could be modeled into the cleft, and crucial active site residues such as Phe18, Lys256, His260, and Thr102 were identified. The molecular basis of the different substrate specificities and different catalytic demands of MtdA compared to methylene-H(4)F dehydrogenases are discussed.
About this Structure
1LUA is a Single protein structure of sequence from Methylobacterium extorquens. Full crystallographic information is available from OCA.
Reference
Structure of methylene-tetrahydromethanopterin dehydrogenase from methylobacterium extorquens AM1., Ermler U, Hagemeier CH, Roth A, Demmer U, Grabarse W, Warkentin E, Vorholt JA, Structure. 2002 Aug;10(8):1127-37. PMID:12176390 Page seeded by OCA on Sat May 3 00:17:44 2008
