8ovw

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'''Unreleased structure'''
 
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The entry 8ovw is ON HOLD until Paper Publication
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==Cryo-EM structure of CBF1-CCAN bound topologically to centromeric DNA==
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<StructureSection load='8ovw' size='340' side='right'caption='[[8ovw]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[8ovw]] is a 11 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8OVW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8OVW FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.4&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ovw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ovw OCA], [https://pdbe.org/8ovw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ovw RCSB], [https://www.ebi.ac.uk/pdbsum/8ovw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ovw ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CBF1_YEAST CBF1_YEAST] Required for chromosome stability and methionine prototrophy. It is involved in chromosomal segregation. Binds to a highly conserved DNA sequence (5'-RTCACRTG-3'), called CDEI, found in centromeres and in several promoters. DNA-binding activity is enhanced by MET28. Required as an auxiliary factor for transcriptional activation of sulfur metabolism together with MET4 and MET28.<ref>PMID:1549123</ref> <ref>PMID:8665859</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The point centromere of budding yeast specifies assembly of the large kinetochore complex to mediate chromatid segregation. Kinetochores comprise the centromere-associated inner kinetochore (CCAN) complex and the microtubule-binding outer kinetochore KNL1-MIS12-NDC80 (KMN) network. The budding yeast inner kinetochore also contains the DNA binding centromere-binding factor 1 (CBF1) and CBF3 complexes. We determined the cryo-electron microscopy structure of the yeast inner kinetochore assembled onto the centromere-specific centromere protein A nucleosomes (CENP-A(Nuc)). This revealed a central CENP-A(Nuc) with extensively unwrapped DNA ends. These free DNA duplexes bind two CCAN protomers, one of which entraps DNA topologically, positioned on the centromere DNA element I (CDEI) motif by CBF1. The two CCAN protomers are linked through CBF3 forming an arch-like configuration. With a structural mechanism for how CENP-A(Nuc) can also be linked to KMN involving only CENP-QU, we present a model for inner kinetochore assembly onto a point centromere and how it organizes the outer kinetochore for chromosome attachment to the mitotic spindle.
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Authors: Dendooven, T.D., Zhang, Z., Yang, J., McLaughlin, S., Schwabb, J., Scheres, S., Yatskevich, S., Barford, D.
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Cryo-EM structure of the complete inner kinetochore of the budding yeast point centromere.,Dendooven T, Zhang Z, Yang J, McLaughlin SH, Schwab J, Scheres SHW, Yatskevich S, Barford D Sci Adv. 2023 Jul 28;9(30):eadg7480. doi: 10.1126/sciadv.adg7480. Epub 2023 Jul , 28. PMID:37506202<ref>PMID:37506202</ref>
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Description: Cryo-EM structure of CBF1-CCAN bound topologically to centromeric DNA
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Barford, D]]
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<div class="pdbe-citations 8ovw" style="background-color:#fffaf0;"></div>
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[[Category: Mclaughlin, S]]
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== References ==
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[[Category: Yang, J]]
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<references/>
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[[Category: Zhang, Z]]
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__TOC__
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[[Category: Schwabb, J]]
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</StructureSection>
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[[Category: Yatskevich, S]]
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[[Category: Large Structures]]
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[[Category: Dendooven, T.D]]
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[[Category: Saccharomyces cerevisiae]]
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[[Category: Scheres, S]]
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[[Category: Barford D]]
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[[Category: Dendooven TD]]
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[[Category: McLaughlin S]]
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[[Category: Scheres S]]
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[[Category: Schwabb J]]
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[[Category: Yang J]]
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[[Category: Yatskevich S]]
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[[Category: Zhang Z]]

Revision as of 05:31, 9 August 2023

Cryo-EM structure of CBF1-CCAN bound topologically to centromeric DNA

PDB ID 8ovw

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