1luh
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1luh.jpg|left|200px]] | [[Image:1luh.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1luh", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_1luh| PDB=1luh | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''SOLUTION NMR STRUCTURE OF SELF-COMPLIMENTARY DUPLEX 5'-D(TCCG*CGGA)2 CONTAINING A TRIMETHYLENE CROSSLINK AT THE N2 POSITION OF G*''' | '''SOLUTION NMR STRUCTURE OF SELF-COMPLIMENTARY DUPLEX 5'-D(TCCG*CGGA)2 CONTAINING A TRIMETHYLENE CROSSLINK AT THE N2 POSITION OF G*''' | ||
| Line 19: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LUH OCA]. | |
==Reference== | ==Reference== | ||
NMR determination of the conformation of a trimethylene interstrand cross-link in an oligodeoxynucleotide duplex containing a 5'-d(GpC) motif., Dooley PA, Zhang M, Korbel GA, Nechev LV, Harris CM, Stone MP, Harris TM, J Am Chem Soc. 2003 Jan 8;125(1):62-72. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12515507 12515507] | NMR determination of the conformation of a trimethylene interstrand cross-link in an oligodeoxynucleotide duplex containing a 5'-d(GpC) motif., Dooley PA, Zhang M, Korbel GA, Nechev LV, Harris CM, Stone MP, Harris TM, J Am Chem Soc. 2003 Jan 8;125(1):62-72. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12515507 12515507] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Dooley, P D.]] | [[Category: Dooley, P D.]] | ||
[[Category: Harris, C M.]] | [[Category: Harris, C M.]] | ||
| Line 31: | Line 27: | ||
[[Category: Stone, M P.]] | [[Category: Stone, M P.]] | ||
[[Category: Zhang, M.]] | [[Category: Zhang, M.]] | ||
| - | [[Category: | + | [[Category: Dna duplex]] |
| - | [[Category: | + | [[Category: Guanine n2-guanine n2 interstrand crosslink]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:18:10 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 21:18, 2 May 2008
SOLUTION NMR STRUCTURE OF SELF-COMPLIMENTARY DUPLEX 5'-D(TCCG*CGGA)2 CONTAINING A TRIMETHYLENE CROSSLINK AT THE N2 POSITION OF G*
Overview
Malondialdehyde interstrand cross-links in DNA show strong preference for 5'-d(CpG) sequences. The cross-links are unstable and a trimethylene cross-link has been used as a surrogate for structural studies. A previous structural study of the 5'-d(CpG) cross-link in the sequence 5'-d(AGGCGCCT), where G is the modified nucleotide, by NMR spectroscopy and molecular dynamics using a simulated annealing protocol showed the guanine residues and the tether lay approximately in a plane such that the trimethylene tether and probably the malondialdehyde tether, as well, could be accommodated without major disruptions of duplex structure [Dooley et al. J. Am Chem. Soc. 2001, 123, 1730-1739]. The trimethylene cross-link has now been studied in a GpC motif using the reverse sequence. The structure lacks the planarity seen with the 5'-d(CpG) sequence and is skewed about the trimethylene cross-link. Melting studies indicate that the trimethylene cross-link is thermodynamically less stable in the GpC motif than in the 5-d(CpG). Furthermore, lack of planarity of the GpC cross-link precludes making an isosteric replacement of the trimethylene tether by malondialdehyde. A similar argument can be used to explain the 5'-d(CpG) preference for interchain cross-linking by acrolein.
About this Structure
Full crystallographic information is available from OCA.
Reference
NMR determination of the conformation of a trimethylene interstrand cross-link in an oligodeoxynucleotide duplex containing a 5'-d(GpC) motif., Dooley PA, Zhang M, Korbel GA, Nechev LV, Harris CM, Stone MP, Harris TM, J Am Chem Soc. 2003 Jan 8;125(1):62-72. PMID:12515507 Page seeded by OCA on Sat May 3 00:18:10 2008
