1lv2

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[[Image:1lv2.gif|left|200px]]
[[Image:1lv2.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1lv2 |SIZE=350|CAPTION= <scene name='initialview01'>1lv2</scene>, resolution 2.7&Aring;
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The line below this paragraph, containing "STRUCTURE_1lv2", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= HNF4G ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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-->
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|DOMAIN=
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{{STRUCTURE_1lv2| PDB=1lv2 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lv2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lv2 OCA], [http://www.ebi.ac.uk/pdbsum/1lv2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1lv2 RCSB]</span>
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}}
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'''Hepatocyte Nuclear Factor 4 is a Transcription Factor that Constitutively Binds Fatty Acids'''
'''Hepatocyte Nuclear Factor 4 is a Transcription Factor that Constitutively Binds Fatty Acids'''
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[[Category: Willson, T M.]]
[[Category: Willson, T M.]]
[[Category: Wisely, B.]]
[[Category: Wisely, B.]]
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[[Category: diabetes]]
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[[Category: Diabetes]]
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[[Category: fatty acid]]
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[[Category: Fatty acid]]
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[[Category: hnf4]]
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[[Category: Hnf4]]
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[[Category: mody]]
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[[Category: Mody]]
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[[Category: nuclear receptor]]
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[[Category: Nuclear receptor]]
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[[Category: transcription factor]]
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[[Category: Transcription factor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:19:14 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:07:44 2008''
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Revision as of 21:19, 2 May 2008

Image:1lv2.gif

Template:STRUCTURE 1lv2

Hepatocyte Nuclear Factor 4 is a Transcription Factor that Constitutively Binds Fatty Acids


Overview

The 2.7 A X-ray crystal structure of the HNF4gamma ligand binding domain (LBD) revealed the presence of a fatty acid within the pocket, with the AF2 helix in a conformation characteristic of a transcriptionally active nuclear receptor. GC/MS and NMR analysis of chloroform/methanol extracts from purified HNF4alpha and HNF4gamma LBDs identified mixtures of saturated and cis-monounsaturated C14-18 fatty acids. The purified HNF4 LBDs interacted with nuclear receptor coactivators, and both HNF4 subtypes show high constitutive activity in transient transfection assays, which was reduced by mutations designed to interfere with fatty acid binding. The endogenous fatty acids did not readily exchange with radiolabeled palmitic acid, and all attempts to displace them without denaturing the protein failed. Our results suggest that the HNF4s may be transcription factors that are constitutively bound to fatty acids.

About this Structure

1LV2 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Hepatocyte nuclear factor 4 is a transcription factor that constitutively binds fatty acids., Wisely GB, Miller AB, Davis RG, Thornquest AD Jr, Johnson R, Spitzer T, Sefler A, Shearer B, Moore JT, Miller AB, Willson TM, Williams SP, Structure. 2002 Sep;10(9):1225-34. PMID:12220494 Page seeded by OCA on Sat May 3 00:19:14 2008

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