1lpj
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(New page: 200px<br /> <applet load="1lpj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lpj, resolution 2.00Å" /> '''Human cRBP IV'''<br...)
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Revision as of 15:56, 12 November 2007
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Human cRBP IV
Overview
Three cellular retinol-binding protein (CRBP) types (CRBP I, II, and III), with distinct tissue distributions and retinoid binding properties have, been structurally characterized thus far. A human binding protein, whose, mRNA is expressed primarily in kidney, heart, and transverse colon, is, shown here to be a CRBP family member (human CRBP IV), according to amino, acid sequence, phylogenetic analysis, gene structure organization, and, x-ray structural analysis. Retinol binding to CRBP IV leads to an, absorption spectrum distinct from a typical holo-CRBP spectrum and is, characterized by an affinity (K(d) = approximately 200 nm) lower than, those for CRBP I, II, and III, as established in direct and competitive, binding assays. As revealed by mutagenic analysis, the presence in CRBP IV, of His(108) in place of Gln(108) is not responsible for the unusual, holo-CRBP IV spectrum. The 2-A resolution crystal structure of human, apo-CRBP IV is very similar to those of other structurally characterized, CRBPs. The side chain of Tyr(60) is present within the binding cavity of, the apoprotein and might affect the interaction with the retinol molecule., These results indicate that human CRBP IV belongs to a clearly distinct, CRBP subfamily and suggest a relatively different mode of retinol binding, for this binding protein.
About this Structure
1LPJ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Ligand binding and structural analysis of a human putative cellular retinol-binding protein., Folli C, Calderone V, Ramazzina I, Zanotti G, Berni R, J Biol Chem. 2002 Nov 1;277(44):41970-7. Epub 2002 Aug 9. PMID:12177003
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