1lvr
From Proteopedia
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[[Image:1lvr.gif|left|200px]] | [[Image:1lvr.gif|left|200px]] | ||
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'''IC3 of CB1 (L431A,A432L) Bound to G(alpha)i''' | '''IC3 of CB1 (L431A,A432L) Bound to G(alpha)i''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1LVR is a [[Single protein]] structure | + | 1LVR is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LVR OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Ulfers, A L.]] | [[Category: Ulfers, A L.]] | ||
[[Category: Wang, L.]] | [[Category: Wang, L.]] | ||
| - | [[Category: | + | [[Category: Alpha domain of g protein i]] |
| - | [[Category: | + | [[Category: Transferred no]] |
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Revision as of 21:20, 2 May 2008
IC3 of CB1 (L431A,A432L) Bound to G(alpha)i
Overview
The structure of the C-terminal region of the third cytoplasmic loop (IC3) of the cannabinoid receptor one (CB1) bound to G(alphai1) has been determined using transferred nuclear Overhauser effects (NOEs). The wild-type IC3 sequence is helical when associated with G(alphai1). In contrast, a peptide containing the amino-acid inversion, Ala(341)-Leu(342) adopts a single turn. These findings correlate with the attenuated G(i) association of CB1 with the Ala(341)-Leu(342) mutation previously observed in vivo and the diminished stimulation of G(alphai1) GTPase activity by the corresponding peptide demonstrated in vitro here. These results, the first to report the structure of a GPCR domain while associated with G protein, imply the C-terminus of CB1 IC3, a region with high-sequence conservation among G-protein coupled receptors, must be helical for efficient coupling and activation of the G(i) protein.
About this Structure
1LVR is a Single protein structure. Full crystallographic information is available from OCA.
Reference
Cannabinoid receptor-G protein interactions: G(alphai1)-bound structures of IC3 and a mutant with altered G protein specificity., Ulfers AL, McMurry JL, Miller A, Wang L, Kendall DA, Mierke DF, Protein Sci. 2002 Oct;11(10):2526-31. PMID:12237474 Page seeded by OCA on Sat May 3 00:20:43 2008
