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1ly1

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[[Image:1ly1.jpg|left|200px]]
[[Image:1ly1.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1ly1 |SIZE=350|CAPTION= <scene name='initialview01'>1ly1</scene>, resolution 2.00&Aring;
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The line below this paragraph, containing "STRUCTURE_1ly1", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Polynucleotide_5'-hydroxy-kinase Polynucleotide 5'-hydroxy-kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.78 2.7.1.78] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= PSET ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 Enterobacteria phage T4])
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-->
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|DOMAIN=
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{{STRUCTURE_1ly1| PDB=1ly1 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ly1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ly1 OCA], [http://www.ebi.ac.uk/pdbsum/1ly1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ly1 RCSB]</span>
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'''Structure and Mechanism of T4 Polynucleotide Kinase'''
'''Structure and Mechanism of T4 Polynucleotide Kinase'''
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[[Category: Shuman, S.]]
[[Category: Shuman, S.]]
[[Category: Wang, L K.]]
[[Category: Wang, L K.]]
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[[Category: kinase]]
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[[Category: Kinase]]
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[[Category: phage]]
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[[Category: Phage]]
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[[Category: phosphatase]]
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[[Category: Phosphatase]]
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[[Category: pnk]]
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[[Category: Pnk]]
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[[Category: polynucleotide]]
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[[Category: Polynucleotide]]
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[[Category: t4]]
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[[Category: T4]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:24:40 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:08:44 2008''
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Revision as of 21:24, 2 May 2008

Image:1ly1.jpg

Template:STRUCTURE 1ly1

Structure and Mechanism of T4 Polynucleotide Kinase


Overview

T4 polynucleotide kinase (Pnk), in addition to being an invaluable research tool, exemplifies a family of bifunctional enzymes with 5'-kinase and 3'-phosphatase activities that play key roles in RNA and DNA repair. T4 Pnk is a homotetramer composed of a C-terminal phosphatase domain and an N-terminal kinase domain. The 2.0 A crystal structure of the isolated kinase domain highlights a tunnel-like active site through the heart of the enzyme, with an entrance on the 5' OH acceptor side that can accommodate a single-stranded polynucleotide. The active site is composed of essential side chains that coordinate the beta phosphate of the NTP donor and the 3' phosphate of the 5' OH acceptor, plus a putative general acid that activates the 5' OH. The structure rationalizes the different specificities of T4 and eukaryotic Pnk and suggests a model for the assembly of the tetramer.

About this Structure

1LY1 is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.

Reference

Structure and mechanism of T4 polynucleotide kinase: an RNA repair enzyme., Wang LK, Lima CD, Shuman S, EMBO J. 2002 Jul 15;21(14):3873-80. PMID:12110598 Page seeded by OCA on Sat May 3 00:24:40 2008

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