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| | <SX load='5h1q' size='340' side='right' viewer='molstar' caption='[[5h1q]], [[Resolution|resolution]] 3.30Å' scene=''> | | <SX load='5h1q' size='340' side='right' viewer='molstar' caption='[[5h1q]], [[Resolution|resolution]] 3.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5h1q]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Caeel Caeel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H1Q OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5H1Q FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5h1q]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H1Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5H1Q FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5h1r|5h1r]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.3Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">inx-6, opu-6, C36H8.2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 CAEEL])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5h1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h1q OCA], [https://pdbe.org/5h1q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5h1q RCSB], [https://www.ebi.ac.uk/pdbsum/5h1q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5h1q ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5h1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h1q OCA], [http://pdbe.org/5h1q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5h1q RCSB], [http://www.ebi.ac.uk/pdbsum/5h1q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5h1q ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/INX6_CAEEL INX6_CAEEL]] Structural component of the gap junctions.[UniProtKB:O61715] | + | [https://www.uniprot.org/uniprot/INX6_CAEEL INX6_CAEEL] Structural component of the gap junctions.[UniProtKB:O61715] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </SX> | | </SX> |
| - | [[Category: Caeel]] | + | [[Category: Caenorhabditis elegans]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Fujiyoshi, Y]] | + | [[Category: Fujiyoshi Y]] |
| - | [[Category: Oshima, A]] | + | [[Category: Oshima A]] |
| - | [[Category: Tani, K]] | + | [[Category: Tani K]] |
| - | [[Category: Gap junction channel]]
| + | |
| - | [[Category: Innexin]]
| + | |
| - | [[Category: Octamer]]
| + | |
| - | [[Category: Transport protein]]
| + | |
| - | [[Category: Wild type]]
| + | |
| Structural highlights
Function
INX6_CAEEL Structural component of the gap junctions.[UniProtKB:O61715]
Publication Abstract from PubMed
Innexins, a large protein family comprising invertebrate gap junction channels, play an essential role in nervous system development and electrical synapse formation. Here we report the cryo-electron microscopy structures of Caenorhabditis elegans innexin-6 (INX-6) gap junction channels at atomic resolution. We find that the arrangements of the transmembrane helices and extracellular loops of the INX-6 monomeric structure are highly similar to those of connexin-26 (Cx26), despite the lack of significant sequence similarity. The INX-6 gap junction channel comprises hexadecameric subunits but reveals the N-terminal pore funnel, consistent with Cx26. The helix-rich cytoplasmic loop and C-terminus are intercalated one-by-one through an octameric hemichannel, forming a dome-like entrance that interacts with N-terminal loops in the pore. These observations suggest that the INX-6 cytoplasmic domains are cooperatively associated with the N-terminal funnel conformation, and an essential linkage of the N-terminal with channel activity is presumably preserved across gap junction families.
Atomic structure of the innexin-6 gap junction channel determined by cryo-EM.,Oshima A, Tani K, Fujiyoshi Y Nat Commun. 2016 Dec 1;7:13681. doi: 10.1038/ncomms13681. PMID:27905396[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Oshima A, Tani K, Fujiyoshi Y. Atomic structure of the innexin-6 gap junction channel determined by cryo-EM. Nat Commun. 2016 Dec 1;7:13681. doi: 10.1038/ncomms13681. PMID:27905396 doi:http://dx.doi.org/10.1038/ncomms13681
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