1lvr
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(New page: 200px<br /> <applet load="1lvr" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lvr" /> '''IC3 of CB1 (L431A,A432L) Bound to G(alpha)i...)
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Revision as of 15:58, 12 November 2007
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IC3 of CB1 (L431A,A432L) Bound to G(alpha)i
Overview
The structure of the C-terminal region of the third cytoplasmic loop (IC3), of the cannabinoid receptor one (CB1) bound to G(alphai1) has been, determined using transferred nuclear Overhauser effects (NOEs). The, wild-type IC3 sequence is helical when associated with G(alphai1). In, contrast, a peptide containing the amino-acid inversion, Ala(341)-Leu(342), adopts a single turn. These findings correlate with the attenuated G(i), association of CB1 with the Ala(341)-Leu(342) mutation previously observed, in vivo and the diminished stimulation of G(alphai1) GTPase activity by, the corresponding peptide demonstrated in vitro here. These results, the, first to report the structure of a GPCR domain while associated with G, protein, imply the C-terminus of CB1 IC3, a region with high-sequence, conservation among G-protein coupled receptors, must be helical for, efficient coupling and activation of the G(i) protein.
About this Structure
1LVR is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
Cannabinoid receptor-G protein interactions: G(alphai1)-bound structures of IC3 and a mutant with altered G protein specificity., Ulfers AL, McMurry JL, Miller A, Wang L, Kendall DA, Mierke DF, Protein Sci. 2002 Oct;11(10):2526-31. PMID:12237474
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