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| | <StructureSection load='5hdj' size='340' side='right'caption='[[5hdj]], [[Resolution|resolution]] 1.89Å' scene=''> | | <StructureSection load='5hdj' size='340' side='right'caption='[[5hdj]], [[Resolution|resolution]] 1.89Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5hdj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_14581 Atcc 14581]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HDJ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5HDJ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5hdj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Priestia_megaterium Priestia megaterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HDJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HDJ FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.89Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">nfrA1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1404 ATCC 14581])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5hdj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hdj OCA], [http://pdbe.org/5hdj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hdj RCSB], [http://www.ebi.ac.uk/pdbsum/5hdj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hdj ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hdj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hdj OCA], [https://pdbe.org/5hdj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hdj RCSB], [https://www.ebi.ac.uk/pdbsum/5hdj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hdj ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A0K0VJM9_PRIMG A0A0K0VJM9_PRIMG] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | | | |
| | ==See Also== | | ==See Also== |
| - | *[[Nitroreductase|Nitroreductase]] | + | *[[Nitroreductase 3D structures|Nitroreductase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 14581]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Morera, S]] | + | [[Category: Priestia megaterium]] |
| - | [[Category: Vigouroux, A]] | + | [[Category: Morera S]] |
| - | [[Category: Dimer]] | + | [[Category: Vigouroux A]] |
| - | [[Category: Nitroreductase enzyme]]
| + | |
| - | [[Category: Oxidoreductase]]
| + | |
| Structural highlights
Function
A0A0K0VJM9_PRIMG
Publication Abstract from PubMed
Mesotrione is a selective herbicide belonging to the triketone family commonly used on maize cultures since 2003. A mesotrione-transforming Bacillus megateriumMes11 strain isolated from an agricultural soil was used as a model to identify the key enzymes initiating the biotransformation of this herbicide. Two enzymes (called NfrA1 and NfrA2/YcnD) were identified and functionally and structurally characterized. Both belong to the NfsA FRP family of the Nitro FMN reductase superfamily (Type I oxygen-insensitive nitroreductase) and show optimal pH and temperature of 6-6.5 and 23-25 degrees C, respectively. Both undergo a ping-pong Bi-Bi mechanism, with NADPH and NADPH/NADH as cofactors for NfrA1 and NfrA2/YcnD respectively. Interestingly, both are also able to reduce various nitro-compounds including pesticides, antibiotics, one prodrug and 4-methylsulfonyl-2-nitrobenzoic acid, one of the mesotrione metabolites retrieved in the environment. This study constitutes the first identification of mesotrione-transforming enzymes. These enzymes (or their corresponding genes) could be used as biomarkers to predict the capacity of ecosystems to transform mesotrione and to assess their contamination both by the parent molecule and/or by the metabolites.
Functional and structural characterization of two Bacillus megaterium nitroreductases biotransforming the herbicide mesotrione.,Carles L, Besse-Hoggan P, Joly M, Vigouroux A, Morera S, Batisson I Biochem J. 2016 Mar 22. pii: BJ20151366. PMID:27005432[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Carles L, Besse-Hoggan P, Joly M, Vigouroux A, Morera S, Batisson I. Functional and structural characterization of two Bacillus megaterium nitroreductases biotransforming the herbicide mesotrione. Biochem J. 2016 Mar 22. pii: BJ20151366. PMID:27005432 doi:http://dx.doi.org/10.1042/BJ20151366
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