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| | <StructureSection load='5hjz' size='340' side='right'caption='[[5hjz]], [[Resolution|resolution]] 1.98Å' scene=''> | | <StructureSection load='5hjz' size='340' side='right'caption='[[5hjz]], [[Resolution|resolution]] 1.98Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5hjz]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HJZ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5HJZ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5hjz]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] and [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HJZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HJZ FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DU:2-DEOXYURIDINE-5-MONOPHOSPHATE'>DU</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.976Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5hkc|5hkc]], [[5hk0|5hk0]], [[5hk3|5hk3]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hjz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hjz OCA], [https://pdbe.org/5hjz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hjz RCSB], [https://www.ebi.ac.uk/pdbsum/5hjz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hjz ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mazF9, mazF-mt1, Rv2801c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5hjz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hjz OCA], [http://pdbe.org/5hjz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hjz RCSB], [http://www.ebi.ac.uk/pdbsum/5hjz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hjz ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MAZF9_MYCTU MAZF9_MYCTU]] Toxic component of a type II toxin-antitoxin (TA) module. Upon expression in E.coli and M.smegmatis inhibits cell growth and colony formation. Its toxic effect is neutralized by coexpression with cognate antitoxin MazE9. Acts as an mRNA interferase, specifically cleaving between U and C in UAC sequences. May cleave its cognate antitoxin's gene (PubMed:25608501). In E.coli expression with non-cognate antitoxins VapB27 and VapB40 partially neutralizes the toxin.<ref>PMID:16611633</ref> <ref>PMID:19016878</ref> <ref>PMID:20011113</ref> <ref>PMID:20876537</ref> <ref>PMID:25608501</ref> | + | [https://www.uniprot.org/uniprot/MAZF9_MYCTU MAZF9_MYCTU] Toxic component of a type II toxin-antitoxin (TA) module. Upon expression in E.coli and M.smegmatis inhibits cell growth and colony formation. Its toxic effect is neutralized by coexpression with cognate antitoxin MazE9. Acts as an mRNA interferase, specifically cleaving between U and C in UAC sequences. May cleave its cognate antitoxin's gene (PubMed:25608501). In E.coli expression with non-cognate antitoxins VapB27 and VapB40 partially neutralizes the toxin.<ref>PMID:16611633</ref> <ref>PMID:19016878</ref> <ref>PMID:20011113</ref> <ref>PMID:20876537</ref> <ref>PMID:25608501</ref> |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Myctu]] | + | [[Category: Mycobacterium tuberculosis]] |
| - | [[Category: Brennan, R G]] | + | [[Category: Mycobacterium tuberculosis H37Rv]] |
| - | [[Category: Yen, T J]] | + | [[Category: Brennan RG]] |
| - | [[Category: Hydrolase-rna complex]] | + | [[Category: Yen TJ]] |
| - | [[Category: Mazf]]
| + | |
| - | [[Category: Toxin-antitoxin system]]
| + | |
| Structural highlights
Function
MAZF9_MYCTU Toxic component of a type II toxin-antitoxin (TA) module. Upon expression in E.coli and M.smegmatis inhibits cell growth and colony formation. Its toxic effect is neutralized by coexpression with cognate antitoxin MazE9. Acts as an mRNA interferase, specifically cleaving between U and C in UAC sequences. May cleave its cognate antitoxin's gene (PubMed:25608501). In E.coli expression with non-cognate antitoxins VapB27 and VapB40 partially neutralizes the toxin.[1] [2] [3] [4] [5]
References
- ↑ Zhu L, Zhang Y, Teh JS, Zhang J, Connell N, Rubin H, Inouye M. Characterization of mRNA interferases from Mycobacterium tuberculosis. J Biol Chem. 2006 Jul 7;281(27):18638-43. Epub 2006 Apr 12. PMID:16611633 doi:http://dx.doi.org/10.1074/jbc.M512693200
- ↑ Gupta A. Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. FEMS Microbiol Lett. 2009 Jan;290(1):45-53. doi:, 10.1111/j.1574-6968.2008.01400.x. Epub 2008 Nov 10. PMID:19016878 doi:http://dx.doi.org/10.1111/j.1574-6968.2008.01400.x
- ↑ Ramage HR, Connolly LE, Cox JS. Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genet. 2009 Dec;5(12):e1000767. doi: 10.1371/journal.pgen.1000767. Epub 2009, Dec 11. PMID:20011113 doi:http://dx.doi.org/10.1371/journal.pgen.1000767
- ↑ Zhu L, Sharp JD, Kobayashi H, Woychik NA, Inouye M. Noncognate Mycobacterium tuberculosis toxin-antitoxins can physically and functionally interact. J Biol Chem. 2010 Dec 17;285(51):39732-8. doi: 10.1074/jbc.M110.163105. Epub 2010, Sep 27. PMID:20876537 doi:http://dx.doi.org/10.1074/jbc.M110.163105
- ↑ Tiwari P, Arora G, Singh M, Kidwai S, Narayan OP, Singh R. MazF ribonucleases promote Mycobacterium tuberculosis drug tolerance and virulence in guinea pigs. Nat Commun. 2015 Jan 22;6:6059. doi: 10.1038/ncomms7059. PMID:25608501 doi:http://dx.doi.org/10.1038/ncomms7059
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