1lww
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(New page: 200px<br /> <applet load="1lww" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lww, resolution 2.1Å" /> '''Borohydride-trapped ...)
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Revision as of 15:58, 12 November 2007
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Borohydride-trapped hOgg1 Intermediate Structure Co-Crystallized with 8-bromoguanine
Contents |
Overview
Most spontaneous damage to bases in DNA is corrected through the action of, the base-excision DNA repair pathway. Base excision repair is initiated by, DNA glycosylases, lesion-specific enzymes that intercept aberrant bases in, DNA and catalyze their excision. How such proteins accomplish the feat of, catalyzing no fewer than five sequential reaction steps using a single, active site has been unknown. To help answer this, we report the structure, of a trapped catalytic intermediate in DNA repair by human 8-oxoguanine, DNA glycosylase. This structure and supporting biochemical results reveal, that the enzyme sequesters the excised lesion base and exploits it as a, cofactor to participate in catalysis. To our knowledge, the present, example represents the first documented case of product-assisted catalysis, in an enzyme-catalyzed reaction.
Disease
Known disease associated with this structure: Renal cell carcinoma, clear cell, somatic OMIM:[601982]
About this Structure
1LWW is a Single protein structure of sequence from Homo sapiens with CA and BRG as ligands. Full crystallographic information is available from OCA.
Reference
Product-assisted catalysis in base-excision DNA repair., Fromme JC, Bruner SD, Yang W, Karplus M, Verdine GL, Nat Struct Biol. 2003 Mar;10(3):204-11. PMID:12592398
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