1lz9

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[[Image:1lz9.gif|left|200px]]
[[Image:1lz9.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1lz9 |SIZE=350|CAPTION= <scene name='initialview01'>1lz9</scene>, resolution 1.70&Aring;
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The line below this paragraph, containing "STRUCTURE_1lz9", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1lz9| PDB=1lz9 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lz9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lz9 OCA], [http://www.ebi.ac.uk/pdbsum/1lz9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1lz9 RCSB]</span>
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}}
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'''ANOMALOUS SIGNAL OF SOLVENT BROMINES USED FOR PHASING OF LYSOZYME'''
'''ANOMALOUS SIGNAL OF SOLVENT BROMINES USED FOR PHASING OF LYSOZYME'''
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[[Category: Dauter, M.]]
[[Category: Dauter, M.]]
[[Category: Dauter, Z.]]
[[Category: Dauter, Z.]]
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[[Category: anomalous dispersion]]
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[[Category: Anomalous dispersion]]
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[[Category: lysozyme]]
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[[Category: Lysozyme]]
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[[Category: single wavelength]]
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[[Category: Single wavelength]]
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[[Category: solvent bromide]]
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[[Category: Solvent bromide]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:26:42 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:09:11 2008''
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Revision as of 21:26, 2 May 2008

Image:1lz9.gif

Template:STRUCTURE 1lz9

ANOMALOUS SIGNAL OF SOLVENT BROMINES USED FOR PHASING OF LYSOZYME


Overview

The anomalous signal of bromide ions, present in the crystal structure of tetragonal hen egg-white lysozyme through the substitution of NaCl by NaBr in the crystallization medium, was used for phasing of X-ray data collected to 1.7 A resolution with a wavelength near the absorption edge of bromine. Phasing of a single wavelength data set, based purely on anomalous deltaf " contribution, led to easily interpretable electron density, equivalent to the complete multiwavelength anonalous dispersion phasing based on four-wavelength data. The classic small-structure direct methods program SHELXS run against all anomalous differences gave a successful solution of six highest peaks corresponding to six bromide ions in the structure with data limited up to a resolution of 3.5 A. Interpretable maps were obtained at a resolution up to 3.0 A using programs MLPHARE and DM. Bromide ions occupy well ordered positions at the protein surface. Phasing based on the single wavelength signal of anomalous scatterers introduced into the ordered solvent shell can be proposed as a tool for solving structures of well diffracting crystals.

About this Structure

1LZ9 is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.

Reference

Anomalous signal of solvent bromides used for phasing of lysozyme., Dauter Z, Dauter M, J Mol Biol. 1999 May 28;289(1):93-101. PMID:10339408 Page seeded by OCA on Sat May 3 00:26:42 2008

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