7yv3
From Proteopedia
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m (Protected "7yv3" [edit=sysop:move=sysop]) |
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| - | '''Unreleased structure''' | ||
| - | + | ==genetically encoded pH sensor Lime at pH10== | |
| + | <StructureSection load='7yv3' size='340' side='right'caption='[[7yv3]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7yv3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YV3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YV3 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7yv3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7yv3 OCA], [https://pdbe.org/7yv3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7yv3 RCSB], [https://www.ebi.ac.uk/pdbsum/7yv3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7yv3 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Genetically encoded pH sensors based on fluorescent proteins are valuable tools for the imaging of cellular events that are associated with pH changes, such as exocytosis and endocytosis. Superecliptic pHluorin (SEP) is a pH-sensitive green fluorescent protein (GFP) variant widely used for such applications. Here, we report the rational design, development, structure, and applications of Lime, an improved SEP variant with higher fluorescence brightness and greater pH sensitivity. The X-ray crystal structure of Lime supports the mechanistic rationale that guided the introduction of beneficial mutations. Lime provides substantial improvements relative to SEP for imaging of endocytosis and exocytosis. Furthermore, Lime and its variants are advantageous for a broader range of applications including the detection of synaptic release and neuronal voltage changes. | ||
| - | + | Rational Engineering of an Improved Genetically Encoded pH Sensor Based on Superecliptic pHluorin.,Shen Y, Wen Y, Sposini S, Vishwanath AA, Abdelfattah AS, Schreiter ER, Lemieux MJ, de Juan-Sanz J, Perrais D, Campbell RE ACS Sens. 2023 Jul 23. doi: 10.1021/acssensors.3c00484. PMID:37481776<ref>PMID:37481776</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Campbell | + | <div class="pdbe-citations 7yv3" style="background-color:#fffaf0;"></div> |
| - | [[Category: Lemieux | + | == References == |
| - | [[Category: Shen | + | <references/> |
| - | [[Category: Wen | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Aequorea victoria]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Campbell R]] | ||
| + | [[Category: Lemieux MJ]] | ||
| + | [[Category: Shen Y]] | ||
| + | [[Category: Wen Y]] | ||
Current revision
genetically encoded pH sensor Lime at pH10
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