8pnk

Structural highlights

Function

DED1_YEAST ATP-binding RNA helicase involved in translation initiation. Remodels RNA in response to ADP and ATP concentrations by facilitating disruption, but also formation of RNA duplexes. Has weak ATP-dependent affinity for dsRNA, but strong ATP-dependent affinity for ssRNA. Acts as a virus host factor involved in the replication of the MBV and the L-A viruses by promoting the negative-strand RNA synthesis. May be involved in recognition of the preinitiation complex and DNA binding of the RNA polymerase III and play a role in mRNA splicing.^{[1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [11] [12]}

References

1. ↑ Iost I, Dreyfus M, Linder P. Ded1p, a DEAD-box protein required for translation initiation in Saccharomyces cerevisiae, is an RNA helicase. J Biol Chem. 1999 Jun 18;274(25):17677-83. PMID:10364207 doi:10.1074/jbc.274.25.17677
2. ↑ Noueiry AO, Chen J, Ahlquist P. A mutant allele of essential, general translation initiation factor DED1 selectively inhibits translation of a viral mRNA. Proc Natl Acad Sci U S A. 2000 Nov 21;97(24):12985-90. PMID:11069307 doi:10.1073/pnas.240460897
3. ↑ Kushner DB, Lindenbach BD, Grdzelishvili VZ, Noueiry AO, Paul SM, Ahlquist P. Systematic, genome-wide identification of host genes affecting replication of a positive-strand RNA virus. Proc Natl Acad Sci U S A. 2003 Dec 23;100(26):15764-9. Epub 2003 Dec 11. PMID:14671320 doi:10.1073/pnas.2536857100
4. ↑ Berthelot K, Muldoon M, Rajkowitsch L, Hughes J, McCarthy JE. Dynamics and processivity of 40S ribosome scanning on mRNA in yeast. Mol Microbiol. 2004 Feb;51(4):987-1001. PMID:14763975 doi:10.1046/j.1365-2958.2003.03898.x
5. ↑ Chong JL, Chuang RY, Tung L, Chang TH. Ded1p, a conserved DExD/H-box translation factor, can promote yeast L-A virus negative-strand RNA synthesis in vitro. Nucleic Acids Res. 2004 Apr 2;32(6):2031-8. PMID:15064363 doi:10.1093/nar/gkh519
6. ↑ Cordin O, Tanner NK, Doère M, Linder P, Banroques J. The newly discovered Q motif of DEAD-box RNA helicases regulates RNA-binding and helicase activity. EMBO J. 2004 Jul 7;23(13):2478-87. PMID:15201868 doi:10.1038/sj.emboj.7600272
7. ↑ Yang Q, Jankowsky E. ATP activities by the DEAD-box protein DED1. Biochemistry. 2005 Oct 18;44(41):13591-601. PMID:16216083 doi:10.1021/bi0508946
8. ↑ Banroques J, Cordin O, Doère M, Linder P, Tanner NK. A conserved phenylalanine of motif IV in superfamily 2 helicases is required for cooperative, ATP-dependent binding of RNA substrates in DEAD-box proteins. Mol Cell Biol. 2008 May;28(10):3359-71. PMID:18332124 doi:10.1128/MCB.01555-07
9. ↑ Jamieson DJ, Rahe B, Pringle J, Beggs JD. A suppressor of a yeast splicing mutation (prp8-1) encodes a putative ATP-dependent RNA helicase. Nature. 1991 Feb 21;349(6311):715-7. PMID:1996139 doi:10.1038/349715a0
10. ↑ Thuillier V, Stettler S, Sentenac A, Thuriaux P, Werner M. A mutation in the C31 subunit of Saccharomyces cerevisiae RNA polymerase III affects transcription initiation. EMBO J. 1995 Jan 16;14(2):351-9. PMID:7835345 doi:10.1002/j.1460-2075.1995.tb07009.x
11. ↑ Chuang RY, Weaver PL, Liu Z, Chang TH. Requirement of the DEAD-Box protein ded1p for messenger RNA translation. Science. 1997 Mar 7;275(5305):1468-71. PMID:9045610 doi:10.1126/science.275.5305.1468
12. ↑ de la Cruz J, Iost I, Kressler D, Linder P. The p20 and Ded1 proteins have antagonistic roles in eIF4E-dependent translation in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1997 May 13;94(10):5201-6. PMID:9144215 doi:10.1073/pnas.94.10.5201