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| | <StructureSection load='1ktr' size='340' side='right'caption='[[1ktr]], [[Resolution|resolution]] 2.70Å' scene=''> | | <StructureSection load='1ktr' size='340' side='right'caption='[[1ktr]], [[Resolution|resolution]] 2.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1ktr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KTR OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1KTR FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1ktr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KTR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KTR FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Monoclonal Antibody 3D5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1ktr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ktr OCA], [http://pdbe.org/1ktr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ktr RCSB], [http://www.ebi.ac.uk/pdbsum/1ktr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ktr ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ktr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ktr OCA], [https://pdbe.org/1ktr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ktr RCSB], [https://www.ebi.ac.uk/pdbsum/1ktr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ktr ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Mus musculus]] |
| - | [[Category: Blank, K]] | + | [[Category: Synthetic construct]] |
| - | [[Category: Capitani, G]] | + | [[Category: Blank K]] |
| - | [[Category: Gruetter, M G]] | + | [[Category: Capitani G]] |
| - | [[Category: Honegger, A]] | + | [[Category: Gruetter MG]] |
| - | [[Category: Kaufmann, M]] | + | [[Category: Honegger A]] |
| - | [[Category: Lindner, P]] | + | [[Category: Kaufmann M]] |
| - | [[Category: Plueckthun, A]] | + | [[Category: Lindner P]] |
| - | [[Category: Tschopp, M]] | + | [[Category: Plueckthun A]] |
| - | [[Category: His tag recognition]]
| + | [[Category: Tschopp M]] |
| - | [[Category: Immune system]]
| + | |
| - | [[Category: Immunoglobulin domain]]
| + | |
| - | [[Category: Scfv]]
| + | |
| - | [[Category: Single chain antibody-antigen complex]]
| + | |
| Structural highlights
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The crystal structure of a mutant form of the single-chain fragment (scFv), derived from the monoclonal anti-His tag antibody 3D5, in complex with a hexahistidine peptide has been determined at 2.7 A resolution. The peptide binds to a deep pocket formed at the interface of the variable domains of the light and the heavy chain, mainly through hydrophobic interaction to aromatic residues and hydrogen bonds to acidic residues. The antibody recognizes the C-terminal carboxylate group of the peptide as well as the main chain of the last four residues and the last three imidazole side-chains. The crystals have a solvent content of 77% (v/v) and form 70 A-wide channels that would allow the diffusion of peptides or even small proteins. The anti-His scFv crystals could thus act as a framework for the crystallization of His-tagged target proteins. Designed mutations in framework regions of the scFv lead to high-level expression of soluble protein in the periplasm of Escherichia coli. The recombinant anti-His scFv is a convenient detection tool when fused to alkaline phosphatase. When immobilized on a matrix, the antibody can be used for affinity purification of recombinant proteins carrying a very short tag of just three histidine residues, suitable for crystallization. The experimental structure is now the basis for the design of antibodies with even higher stability and affinity.
Crystal structure of the anti-His tag antibody 3D5 single-chain fragment complexed to its antigen.,Kaufmann M, Lindner P, Honegger A, Blank K, Tschopp M, Capitani G, Pluckthun A, Grutter MG J Mol Biol. 2002 Apr 19;318(1):135-47. PMID:12054774[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Kaufmann M, Lindner P, Honegger A, Blank K, Tschopp M, Capitani G, Pluckthun A, Grutter MG. Crystal structure of the anti-His tag antibody 3D5 single-chain fragment complexed to its antigen. J Mol Biol. 2002 Apr 19;318(1):135-47. PMID:12054774 doi:10.1016/S0022-2836(02)00038-4
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